Local-equilibrium behavior of retained pH and ionic strength gradients in preparative chromatography

被引：18

作者：

Frey, DD

机构：

[1] Department of Chemical Engineering, Univ. of Maryland Baltimore County, Baltimore

来源：

BIOTECHNOLOGY PROGRESS | 1996年 / 12卷 / 01期

关键词：

D O I：

10.1021/bp950061e

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A local-equilibrium theory is developed and used to investigate the behavior of retained pH and ionic strength gradients formed on a strong-base anion-exchange column where both anion- and cation-forming buffering species are present. Analytical relations are presented for several simplified cases of practical interest and are used to illustrate factors governing the selection of the elution and presaturation buffers when retained pH and ionic strength gradients are employed for preparative protein chromatography.

引用

页码：65 / 72

页数：8

共 21 条

[11] CHROMATOPHORESIS - A NEW APPROACH TO THE THEORY AND PRACTICE OF CHROMATOFOCUSING .1. GENERAL-PRINCIPLES [J].