Host-Derived Nitrate Boosts Growth of E. coli in the Inflamed Gut

被引：744

作者：

Winter, Sebastian E. ^{[1
]}

Winter, Maria G. ^{[1
]}

Xavier, Mariana N. ^{[1
]}

Thiennimitr, Parameth ^{[1
,2
]}

Poon, Victor ^{[1
]}

Keestra, A. Marijke ^{[1
]}

Laughlin, Richard C. ^{[3
]}

Gomez, Gabriel ^{[3
]}

Wu, Jing ^{[3
]}

Lawhon, Sara D. ^{[3
]}

Popova, Ina E. ^{[4
]}

Parikh, Sanjai J. ^{[4
]}

Adams, L. Garry ^{[3
]}

Tsolis, Renee M. ^{[1
]}

Stewart, Valley J. ^{[5
]}

Baeumler, Andreas J. ^{[1
]}

机构：

[1] Univ Calif Davis, Sch Med, Dept Med Microbiol & Immunol, Davis, CA 95616 USA

[2] Chiang Mai Univ, Fac Med, Dept Microbiol, Chiang Mai 50000, Thailand

[3] Texas A&M Univ, Coll Vet Med & Biomed Sci, Dept Vet Pathobiol, College Stn, TX USA

[4] Univ Calif Davis, Dept Land Air & Water Resources, Davis, CA 95616 USA

[5] Univ Calif Davis, Coll Biol Sci, Dept Microbiol, Davis, CA 95616 USA

来源：

SCIENCE | 2013年 / 339卷 / 6120期

关键词：

CROHNS-DISEASE; NITRIC-OXIDE; MICROBIOTA; OXIDATION; DISRUPTS; FLORA;

D O I：

10.1126/science.1232467

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Changes in the microbial community structure are observed in individuals with intestinal inflammatory disorders. These changes are often characterized by a depletion of obligate anaerobic bacteria, whereas the relative abundance of facultative anaerobic Enterobacteriaceae increases. The mechanisms by which the host response shapes the microbial community structure, however, remain unknown. We show that nitrate generated as a by-product of the inflammatory response conferred a growth advantage to the commensal bacterium Escherichia coli in the large intestine of mice. Mice deficient in inducible nitric oxide synthase did not support the growth of E. coli by nitrate respiration, suggesting that the nitrate generated during inflammation was host-derived. Thus, the inflammatory host response selectively enhances the growth of commensal Enterobacteriaceae by generating electron acceptors for anaerobic respiration.

引用

页码：708 / 711

页数：4

共 21 条

[1] The mechanism of carbon dioxide catalysis in the hydrogen peroxide n-oxidation of amines [J].

Balagam, Bhararthi ;

Richardson, David E. .

INORGANIC CHEMISTRY, 2008, 47 (03) :1173-1178

[2] Enteric salmonellosis disrupts the microbial ecology of the murine gastrointestinal tract [J].

Barman, Melissa ;

Unold, David ;

Shifley, Kathleen ;

Amir, Elad ;

Hung, Kueichun ;

Bos, Nicolaas ;

Salzman, Nita .

INFECTION AND IMMUNITY, 2008, 76 (03) :907-915

[3] Diversity of the human intestinal microbial flora [J].

Eckburg, PB ;

Bik, EM ;

Bernstein, CN ;

Purdom, E ;

Dethlefsen, L ;

Sargent, M ;

Gill, SR ;

Nelson, KE ;

Relman, DA .

SCIENCE, 2005, 308 (5728) :1635-1638

[4] Rectal nitric oxide gas and stool cytokine levels during the course of infectious gastroenteritis [J].

Enocksson, A ;

Lundberg, J ;

Weitzberg, E ;

Norrby-Teglund, A ;

Svenungsson, B .

CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, 2004, 11 (02) :250-254

[5] Molecular-phylogenetic characterization of microbial community imbalances in human inflammatory bowel diseases [J].

Frank, Daniel N. ;

Amand, Allison L. St. ;

Feldman, Robert A. ;

Boedeker, Edgar C. ;

Harpaz, Noam ;

Pace, Norman R. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (34) :13780-13785

[6] Enterobacteriaceae Act in Concert with the Gut Microbiota to Induce Spontaneous and Maternally Transmitted Colitis [J].

Garrett, Wendy S. ;

Gallini, Carey A. ;

Yatsunenko, Tanya ;

Michaud, Monia ;

DuBois, Andrea ;

Delaney, Mary L. ;

Punit, Shivesh ;

Karlsson, Maria ;

Bry, Lynn ;

Glickman, Jonathan N. ;

Gordon, Jeffrey I. ;

Onderdonk, Andrew B. ;

Glimcher, Laurie H. .

CELL HOST & MICROBE, 2010, 8 (03) :292-300

[7]

Gennis R. B., 1996, CELLULAR MOL BIOL, V1, P217

[8] THE ASSESSMENT OF FECAL FLORA IN PATIENTS WITH INFLAMMATORY BOWEL-DISEASE BY A SIMPLIFIED BACTERIOLOGICAL TECHNIQUE [J].

GIAFFER, MH ;

HOLDSWORTH, CD ;

DUERDEN, BI .

JOURNAL OF MEDICAL MICROBIOLOGY, 1991, 35 (04) :238-243

[9] Inerences between tissue-associated intestinal microfloras of patients with Crohn's disease and ulcerative colitis [J].

Gophna, Uri ;

Sommerfeld, Katrin ;

Gophna, Sharon ;

Doolittle, W. Ford ;

van Zanten, Sander J. O. Veldhuyzen .

JOURNAL OF CLINICAL MICROBIOLOGY, 2006, 44 (11) :4136-4141

[10] Shift Towards Pro-inflammatory Intestinal Bacteria Aggravates Acute Murine Colitis via Toll-like Receptors 2 and 4 [J].

Heimesaat, Markus M. ;

Fischer, Andre ;

Siegmund, Britta ;

Kupz, Andreas ;

Niebergall, Julia ;

Fuchs, David ;

Jahn, Hannah-Katharina ;

Freudenberg, Marina ;

Loddenkemper, Christoph ;

Batra, Arvind ;

Lehr, Hans-Anton ;

Liesenfeld, Oliver ;

Blaut, Michael ;

Goebel, Ulf B. ;

Schumann, Ralf R. ;

Bereswill, Stefan .

PLOS ONE, 2007, 2 (07)

← 1 2 3 →