Enterobacteriaceae Act in Concert with the Gut Microbiota to Induce Spontaneous and Maternally Transmitted Colitis

被引：645

作者：

Garrett, Wendy S. ^{[1
]}

Gallini, Carey A. ^{[1
]}

Yatsunenko, Tanya ^{[3
]}

Michaud, Monia ^{[1
]}

DuBois, Andrea ^{[4
]}

Delaney, Mary L. ^{[4
]}

Punit, Shivesh ^{[1
]}

Karlsson, Maria ^{[3
]}

Bry, Lynn ^{[4
]}

Glickman, Jonathan N. ^{[4
]}

Gordon, Jeffrey I. ^{[3
]}

Onderdonk, Andrew B. ^{[4
]}

Glimcher, Laurie H. ^{[1
,2
,5
]}

机构：

[1] Harvard Univ, Dept Immunol & Infect Dis, Sch Publ Hlth, Boston, MA 02115 USA

[2] Harvard Univ, Dept Med, Sch Med, Boston, MA 02115 USA

[3] Washington Univ, Ctr Genome Sci & Syst Biol, Sch Med, St Louis, MO 63108 USA

[4] Brigham & Womens Hosp, Dept Pathol, Boston, MA 02115 USA

[5] MGH Harvard MIT, Ragon Inst, Boston, MA 02129 USA

来源：

CELL HOST & MICROBE | 2010年 / 8卷 / 03期

关键词：

T-BET DEFICIENCY; IMMUNE-RESPONSES; HOST; MAMMALS; FLORA;

D O I：

10.1016/j.chom.2010.08.004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Disruption of homeostasis between the host immune system and the intestinal microbiota leads to inflammatory bowel disease (IBD). Whether IBD is instigated by individual species or disruptions of entire microbial communities remains controversial. We characterized the fecal microbial communities in the recently described T-bet(-/-) x Rag2(-/-) ulcerative colitis (TRUC) model driven by T-bet deficiency in the innate immune system. 16S rRNA-based analysis of TRUC and Rag2(-/-) mice revealed distinctive communities that correlate with host genotype. The presence of Klebsiella pneumoniae and Proteus mirabilis correlates with colitis in TRUC animals, and these TRUC-derived strains can elicit colitis in Rag2(-/-) and WT adults but require a maternally transmitted endogenous microbial community for maximal intestinal inflammation. Cross-fostering experiments indicated a role for these organisms in maternal transmission of disease. Our findings illustrate how gut microbial communities work in concert with specific culturable colitogenic agents to cause IBD.

引用

页码：292 / 300

页数：9

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