Non-suppressed conductivity and indirect UV detection of carboxylic acids in environmental samples by ion-exclusion chromatography using 2,6-pyridinedicarboxylic acidic eluent

被引：13

作者：

Chen, ZL

Tang, C

Xu, JM

机构：

[1] Univ Western Australia, Ctr Legumes Mediterranean Agr Soil Sci & Plant Nu, Nedlands, WA 6907, Australia

[2] Zhejiang Univ, Dept Soil Sci & Agrochem, Hangzhou 310029, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY A | 1999年 / 859卷 / 02期

关键词：

non-suppressed conductivity detection; detection LC; carboxylic acids; 2,6-pyridinedicarboxylic acid;

D O I：

10.1016/S0021-9673(99)00885-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

2,6-Pyridinedicarboxylic acid (PDCA) was evaluated as an eluent for indirect UV and non-suppressed conductivity detection of carboxylic acids in ion-exclusion chromatography. The effect of PDCA concentration on the separation and detection sensitivity was investigated. The reasonable resolutions between carboxylic acids were achieved using 1 mM PDCA eluent. Detection limits were 1.0-7.0 mu M for conductivity detection and 8-30 mu M for UV detection. Compared to the eluent containing 1 mM sulfuric acid, the method offers a high resolution and high detection sensitivity for both detectors due to its high molar absorptivity and low background conductance. The proposed method was demonstrated to be useful for the determination of carboxylic acids in environmental samples with direct sample injection. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：173 / 181

页数：9

共 14 条

[1] Liquid chromatography of carboxylic acids using potentiometric detection with a tungsten oxide electrode [J].