tRNA-intergenic spacer PCR for the identification of Pasteurella and Mannheimia spp.

被引：24

作者：

Catry, B

Baele, M

Opsomer, G

de Kruif, A

Decostere, A

Haesebrouck, F

机构：

[1] Univ Ghent, Fac Vet Med, Dept Reprod Obstet & Herd Hlth, B-9820 Merelbeke, Belgium

[2] Univ Ghent, Fac Vet Med, Dept Pathol Bacteriol & Poultry Dis, B-9820 Merelbeke, Belgium

来源：

VETERINARY MICROBIOLOGY | 2004年 / 98卷 / 3-4期

关键词：

mannheimia haemolytica; pasteurella multocida; molecular identification; tDNA-PCR;

D O I：

10.1016/j.vetmic.2003.10.027

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

tRNA-intergenic spacer PCR (tDNA-PCR) was evaluated for its effectiveness in differentiating Pasteurella and Mannheimia (sub)species predominantly of ruminant origin. For this purpose, 38 reference strains and 13 field isolates belonging to both genera were investigated. tDNA-PCR enabled discrimination of all Pasteurella species tested (Pasteurella (P) aerogenes, R avium, P. canis, P. lymphangitidis, R multocida, R trehalosi). For the differentiation of the subspecies of P multocida, an additional dulcitol reaction was required. Two of the five so far-defined Mannheimia species, M. granulomatis and M. varigena, had a distinct fingerprinting profile. The remaining three phylogenetically highly related species (M. haemolytica, M. glucosida, and M. ruminalis) clustered together. Nevertheless, M. ruminalis is non-haemolytic, and M. haemolytica and M. glucosida can be differentiated on the basis of two additional phenotypic characteristics (beta-glucosidase and aesculin hydrolysis). In conclusion, tDNA-PCR is a useful tool in differentiating organisms belonging to the genera Pasteurella and Mannheima. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：251 / 260

页数：10

共 30 条

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