Projections of diencephalic dopamine neurons into the spinal cord in mice

被引：91

作者：

Qu, S ^{[1
]}

Ondo, WG ^{[1
]}

Zhang, X ^{[1
]}

Xie, WJ ^{[1
]}

Pan, TH ^{[1
]}

Le, WD ^{[1
]}

机构：

[1] Baylor Coll Med, Dept Neurol, Houston, TX 77030 USA

来源：

EXPERIMENTAL BRAIN RESEARCH | 2006年 / 168卷 / 1-2期

关键词：

diencephalons; dopaminergic; A11; fluoro gold; restless legs syndrome;

D O I：

10.1007/s00221-005-0075-1

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The aim of this study is to investigate the pathway of diencephalic dopaminergic (DA) neuronal innervating into the spinal cord in mice, the pathway is postulated relevant to clinical restless legs syndrome (RLS). Tyrosine hydroxylase (TH) immunohistochemstry was used to identify the DA neuron. The fluorescent tracer Fluoro-Gold (FG) was sterotaxically injected into the T-10-L-5 spinal cord of CBL57 mice (n=20) seven days before the animals were sacrificed. The diencephalic sections were stained with TH antibody and the FG tracer present in the diencephalic DA neurons were examined under fluoresce microscope. The average number of total DA neurons per side in A(11), A(12), A(13) and A(14) was 66 +/- 8, 221 +/- 12, 350 +/- 17 and 254 +/- 21 respectively. After being injected into the spinal cord, FG reached the DA neurons within the A(10) and A(11) groups, but didn't target to any other DA neuron groups including the A(8) and A(9) groups in substantia nigra (SN). The diencephalic A(11) DA neurons possessed long axons extending over several segments and possibly traversing the entire length of the spinal cord. It is the first time to report A(10) and A(11) DA neuron projections into the spinal cord in mice.

引用

页码：152 / 156

页数：5

共 21 条

[11] Clinical, polysomnographic, and genetic characteristics of restless legs syndrome: A study of 133 patients diagnosed with new standard criteria [J].