Prokaryotic transcription regulators: more than just the helix-turn-helix motif

被引:155
作者
Huffman, JL [1 ]
Brennan, RG [1 ]
机构
[1] Oregon Hlth & Sci Univ, Dept Biochem & Mol Biol, Portland, OR 97201 USA
关键词
D O I
10.1016/S0959-440X(02)00295-6
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Over the past two years, the structures of many prokaryotic transcriptional regulators have been solved, and several of them have revealed the structural mechanism of gene regulation. The crystal structure of BmrR-TPP-DNA reveals a novel mechanism of transcription activation, whereby the drug-bound protein activates the bmr promoter by local DNA unwinding and base pair disruption. Myristoyl-CoA induces FadR by a three-helix pushing mechanism, whereas TetR employs a helical pendulum motion to regulate expression. The structures of AbrB, and DNA complexes of Rob and MuR unveil a novel DNA-binding motif, 'the looped-hinge helix, and new uses of the helix-turn-helix and winged helix motifs in DNA binding.
引用
收藏
页码:98 / 106
页数:9
相关论文
共 55 条
[51]   ESCHERICHIA-COLI BIOTIN HOLOENZYME SYNTHETASE BIOREPRESSOR CRYSTAL-STRUCTURE DELINEATES THE BIOTIN-BINDING AND DNA-BINDING DOMAINS [J].
WILSON, KP ;
SHEWCHUK, LM ;
BRENNAN, RG ;
OTSUKA, AJ ;
MATTHEWS, BW .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (19) :9257-9261
[52]   Structural classification of HTH DNA-binding domains and protein-DNA interaction modes [J].
Wintjens, R ;
Rooman, M .
JOURNAL OF MOLECULAR BIOLOGY, 1996, 262 (02) :294-313
[53]  
Wojciak JM, 2001, NAT STRUCT BIOL, V8, P84
[54]   Structural understanding of the allosteric conformational change of cyclic AMP receptor protein by cyclic AMP binding [J].
Won, HS ;
Yamazaki, T ;
Lee, TW ;
Yoon, MK ;
Park, SH ;
Kyogoku, Y ;
Lee, BJ .
BIOCHEMISTRY, 2000, 39 (45) :13953-13962
[55]   The Fadr DNA complex -: Transcriptional control of fatty acid metabolism in Escherichia coli [J].
Xu, YB ;
Heath, RJ ;
Li, ZM ;
Rock, CO ;
White, SW .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (20) :17373-17379