Activation of intrinsic hippocampal theta oscillations by acetylcholine in rat septo-hippocampal cocultures

被引:46
作者
Fischer, Y [1 ]
Gähwiler, BH
Thompson, SM
机构
[1] Univ Zurich, Brain Res Inst, CH-8057 Zurich, Switzerland
[2] Univ Maryland, Sch Med, Dept Physiol, Baltimore, MD 21201 USA
来源
JOURNAL OF PHYSIOLOGY-LONDON | 1999年 / 519卷 / 02期
关键词
D O I
10.1111/j.1469-7793.1999.0405m.x
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
1. Oscillatory electro-encephalographic activity at theta frequencies (4-15 Hz) can be recorded from the hippocampus in vivo and depends on intact septal projections. The hypothesis that these oscillations are imposed on the hippocampus by rhythmically active septal inputs was tested using dual. intracellular recordings from CA1 and CA3 pyramidal cells in septohippocampal cocultures. 2. Septo-hippocampal cocultures displayed spontaneous oscillatory synaptic activity at theta frequencies. In CA3 cells, EPSP/IPSP sequences predominated, whereas only EPSPs were apparent in CA1 cells. Synaptic potentials in CA3 cells preceded those in CA1 cells by 5-10 ms. 3. Oscillatory synaptic activity was blocked in cocultures by the muscarinic antagonist atropine (0.1 mu M), facilitated but unchanged in frequency upon application of the acetylcholinesterase inhibitor neostigmine (1 mu M), and not seen in hippocampal monocultures. 4. The muscarinic agonist methacholine (5-20 nM) induced oscillatory synaptic activity at 4-15 Hz in hippocampal monocultures, which was identical to that occurring spontaneously in septo-hippocampal cocultures. 5. Synaptic theta activity was observed in cocultures of septal tissue with subdissected hippocampal slices containing area CA3 alone, but not in septo-CA1. cocultures. 6. We conclude that oscillatory synaptic activity at theta frequencies, with similar characteristics to theta activity in vivo, can be generated by the hippocampal network in response to activation of muscarinic receptors by synaptically released acetylcholine from septal afferents. Furthermore, the oscillatory activity is determined by mechanisms intrinsic to the hippocampal circuitry, particularly area CA3. Rhythmic septal input is not required.
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页码:405 / 413
页数:9
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