Microfabricated silicon flow-cell for optical monitoring of biological fluids

被引：15

作者：

Chau, LK ^{[1
]}

Osborn, T

Wu, CC

Yager, P

机构：

[1] Univ Washington, Ctr Bioengn, Seattle, WA 98195 USA

[2] Natl Chung Cheng Univ, Dept Chem, Chiayi 621, Taiwan

来源：

ANALYTICAL SCIENCES | 1999年 / 15卷 / 08期

关键词：

optical flow cell; micromachining; albumin;

D O I：

10.2116/analsci.15.721

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The feasibility of the optical monitoring of biological fluids, for example, albumin in serum, in a microfabricated silicon flow cell has been demonstrated. This module couples a fiber-optic reflection probe tone emitting fiber and six receiving fibers) to a small-volume silicon flow-cell (in mu l range) for reflection-absorption measurements. The coupling efficiency of light between the optical fibers in such a system has been calculated (7.07%), and its optimum values (19.5% - 19.6%) as a function of the cover glass thickness and the silicon channel depth predicted.

引用

页码：721 / 724

页数：4

共 10 条

[1]

BRODY JP, IN PRESS BIOPHYS J

[2]

Deming S.N., 1987, Experimental Design: A Chemometric Approach

[3]

Hecht E., 1987, OPTICS, p[94, 282]

[4]

Hermansson K., 1991, TRANSDUCERS '91. 1991 International Conference on Solid-State Sensors and Actuators. Digest of Technical Papers (Cat. No.91CH2817-5), P193, DOI 10.1109/SENSOR.1991.148835

[5] Microfabrication of a planar absorbance and fluorescence cell for integrated capillary electrophoresis devices [J].