Generation of an shRNAi expression library against the whole human transcripts

被引：35

作者：

Miyagishi, M

Matsumoto, S

Taira, K ^{[1
]}

机构：

[1] Univ Tokyo, Sch Engn, Dept Chem & Biotechnol, Tokyo 1138656, Japan

[2] Natl Inst Adv Ind Sci & Technol, Gene Funct Res Ctr, AIST, Tsukuba, Ibaraki 3058562, Japan

[3] Univ Tokyo, Grad Sch Med, Dept Cardiovasc Med, Tokyo 1130033, Japan

来源：

VIRUS RESEARCH | 2004年 / 102卷 / 01期

关键词：

RNA interference; U6; promoter; algorithm; RNAi library;

D O I：

10.1016/j.virusres.2004.01.022

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

RNA interference is a phenomenon in which expression of an individual gene can be specifically silenced by introducing a double-stranded RNA. homologous to the gene, and is receiving attention as a powerful tool for reverse genetics in the post-genome era. Throughout our Current research to generate an siRNA expression library for the whole human genome, we face many technical difficulties. We present here the strategies for overcoming some of the difficulties, including the development of genetically stable and highly active siRNA expression vectors, the selection procedure of the favorable target sites, and the efficient and low cost procedure for constructing an siRNA expression library. Furthermore. we demonstrate that the screening using the constructed siRNA-expression library indeed works, by evaluating siRNA-expression library against apoptosis-related genes. (C) 2004 Published by Elsevier B.V.

引用

页码：117 / 124

页数：8

共 35 条

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