Synergistic effect of gene-electro transfer and adjuvant cytokines in increasing the potency of hepatitis C virus genetic vaccination

被引：8

作者：

Arcuri, Mirko ^{[1
]}

Cappelletti, Manuela ^{[1
]}

Zampaglione, Immacolata ^{[1
]}

Aurisicchio, Luigi ^{[1
]}

Nicosia, Alfredo ^{[1
]}

Ciliberto, Gennaro ^{[1
]}

Fattori, Elena ^{[1
]}

机构：

[1] Ist Ric Biol Mol P Angeletti IRBM, Rome, Italy

来源：

JOURNAL OF GENE MEDICINE | 2008年 / 10卷 / 09期

关键词：

HCV; E2; GET; Cytokines; Vaccination;

D O I：

10.1002/jgm.1217

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Background Gene electro-transfer (GET) increases DNA uptake and expression by muscle cells following intramuscular plasmid injection. This technology has been used to increase the production of therapeutic proteins, such as cytokines and growth factors, and to improve immunization efficiency following the injection of antigen-encoding plasmids. Methods Hepatitis C Virus (HCV) E2 and cytokine encoding plasmids were co-injected in the mouse quadriceps with or without GET and vaccination outcome was monitored by analysis of antigen-specific cellular-mediated or antibody-mediated immunity. Results GET co-injection of cytokine-encoding and HCV E2-encoding plasmids strongly enhanced T- or B-cell responses to various levels, depending on the particular combination used. Conclusions We propose that a cocktail of plasmids followed by GET can be the most efficient and fine-tunable approach for genetic immunization. Copyright (C) 2008 John Wiley & Sons, Ltd.

引用

页码：1048 / 1054

页数：7

共 32 条

[31] MANIPULATION OF THE IMMUNE-RESPONSE TO A PLASMID-ENCODED VIRAL-ANTIGEN BY COINOCULATION WITH PLASMIDS EXPRESSING CYTOKINES [J].