Improved in vivo photoacoustic microscopy based on a virtual-detector concept

被引:163
作者
Li, ML
Zhang, HF
Maslov, K
Stoica, G
Wang, LHV
机构
[1] Texas A&M Univ, Dept Biomed Engn, Opt Imaging Lab, College Stn, TX 77843 USA
[2] Texas A&M Univ, Dept Vet Pathobiol, College Stn, TX 77843 USA
关键词
D O I
10.1364/OL.31.000474
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
Recently an in vivo high-resolution backward-mode photoacoustic microscope was developed that shows potential for applications in dermatology and related cancer research. However, the limited depth of focus of the large-numerical-aperture (NA) ultrasonic lens employed in this system causes the image quality to deteriorate significantly in the out-of-focus region. To solve this problem, we devised and explored, for the first time to our knowledge, a virtual-detector-based synthetic-aperture focusing technique, combined with coherence weighting, for photoacoustic microscopy with such a large-NA transducer. Images of phantoms show that the proposed technique improves the -6 dB lateral resolution from 49-379 to 46-53 mu m and increases the signal-to-noise ratio by up to 29 dB, depending on the distance from the ultrasonic focal point. In vivo experiments show that the technique also provides a clearer representation of the vascular distribution in the rat's scalp. (c) 2006 Optical Society of America.
引用
收藏
页码:474 / 476
页数:3
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