Denaturation and renaturation of self-assembled yeast iso-1-cytochrome c on Au

被引：41

作者：

Chah, S

Kumar, CV

Hammond, MR

Zare, RN ^{[1
]}

机构：

[1] Stanford Univ, Dept Chem, Stanford, CA 94305 USA

[2] Univ Connecticut, Dept Chem, Storrs, CT 06269 USA

来源：

ANALYTICAL CHEMISTRY | 2004年 / 76卷 / 07期

关键词：

D O I：

10.1021/ac035416k

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We have made surface plasmon resonance (SPR) measurements of yeast iso-1-cytochrome c (Cyt c) on a gold surface. Angle-resolved SPR curves are recorded as a function of urea concentration before and after self-assembly of the Cyt c. Exposure to a urea solution causes denaturation of Cyt c, which shifts the minimum in the SPR curve to a larger angle and decreases the signal amplitude. The Gibbs free energy change for denaturation of the protein on Au is calculated from the change of the SPR signal amplitude with urea concentration. We find that (1) Cyt c can be reversibly denatured and renatured, depending on the urea concentration, and (2) the Gibbs free energy change for denaturation of Cyt c on An surface in water, DeltaGdegrees(water), is 1.5 kcal/mol, which is similar to4 times less than that in bulk solution.

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页码：2112 / 2117

页数：6

相关论文

共 36 条

[1] Surface plasmon resonance fibre-optic sensor for gas detection [J].

Abdelghani, A ;

Chovelon, JM ;

JaffrezicRenault, N ;

RonotTrioli, C ;

Veillas, C ;

Gagnaire, H .

SENSORS AND ACTUATORS B-CHEMICAL, 1997, 39 (1-3) :407-410

[2]

ARAKAWA T, 1996, THIN SOLID FILMS, V281, P466

[3] CONFORMATIONAL-CHANGES IN ADSORBED PROTEINS [J].

BALL, A ;

JONES, RAL .

LANGMUIR, 1995, 11 (09) :3542-3548

[4] Enzyme-coated carbon nanotubes as single-molecule biosensors [J].

Besteman, K ;

Lee, JO ;

Wiertz, FGM ;

Heering, HA ;

Dekker, C .

NANO LETTERS, 2003, 3 (06) :727-730

[5] Preparation of a stable biocatalyst of bovine liver catalase using immobilization and postimmobilization techniques [J].

Betancor, L ;

Hidalgo, A ;

Fernández-Lorente, G ;

Mateo, C ;

Fernández-Lafuente, R ;

Guisan, JM .

BIOTECHNOLOGY PROGRESS, 2003, 19 (03) :763-767

[6] The effect of substrate metal on 2-aminoethanethiol and nanoparticle enhanced surface plasmon resonance imaging [J].

Chah, S ;

Hutter, E ;

Roy, D ;

Fendler, JH ;

Yi, J .

CHEMICAL PHYSICS, 2001, 272 (01) :127-136

[7] DETERMINATION OF THICKNESS AND DIELECTRIC-CONSTANT OF THIN TRANSPARENT DIELECTRIC LAYERS USING SURFACE-PLASMON RESONANCE [J].

DEBRUIJN, HE ;

ALTENBURG, BSF ;

KOOYMAN, RPH ;

GREVE, J .

OPTICS COMMUNICATIONS, 1991, 82 (5-6) :425-432

[8] Quantitative measurements and modeling of kinetics in nucleic acid monolayer films using SPR spectroscopy [J].

Georgiadis, R ;

Peterlinz, KP ;

Peterson, AW .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2000, 122 (13) :3166-3173

[9] Refolding kinetics of cytochrome c551 reveals a mechansitic difference between urea and guanidine [J].

Gianni, S ;

Brunori, M ;

Travaglini-Allocatelli, C .

PROTEIN SCIENCE, 2001, 10 (08) :1685-1688

[10] Protein stability: Functional dependence of denaturational Gibbs energy on urea concentration [J].

Gupta, R ;

Ahmad, F .

BIOCHEMISTRY, 1999, 38 (08) :2471-2479

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