Controlled intracellular self-assembly of gadolinium nanoparticles as smart molecular MR contrast agents

被引：87

作者：

Cao, Chun-Yan ^{[2
]}

Shen, Ying-Ying ^{[1
]}

Wang, Jian-Dong ^{[3
]}

Li, Li ^{[1
]}

Liang, Gao-Lin ^{[2
]}

机构：

[1] Sun Yat Sen Univ, Ctr Canc, State Key Lab Oncol S China, Imaging Diag & Intervent Ctr, Guangzhou 510060, Guangdong, Peoples R China

[2] Univ Sci & Technol China, CAS Key Lab Soft Matter Chem, Dept Chem, Hefei 230026, Anhui, Peoples R China

[3] Nanjing Univ, Sch Med, Nanjing Jinling Hosp, Lab Mol Pathol & Mol Imaging,Dept Pathol, Nanjing 210002, Jiangsu, Peoples R China

来源：

SCIENTIFIC REPORTS | 2013年 / 3卷

基金：

中国国家自然科学基金;

关键词：

IN-VIVO; FURIN; EXPRESSION; MYELOPEROXIDASE; AMPLIFICATION; ACTIVATION; GROWTH; SIGNAL;

D O I：

10.1038/srep01024

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Herein we developed a new "smart" Gd-based MR contrast agent (i.e., 1) which is susceptive to furin, a protease overexpressed in tumor. Under the action of furin, 1 condenses to form dimers (1-Ds) and the latter self-assemble into gadolinium nanparticles (Gd-NPs). Relaxivity of 1-D is more than 2 folds of those of 1 and magnevist at 1.5 T, and 1.4 folds of that of 1 at 3 T. Intracellular condensation of 1 in furin-overexpressed MDA-MB-468 cells was proven with direct two-photon laser microscopy (TPLM) fluorescence imaging of the cells incubated with the europium analog of 1 (i.e., 2). Intracellular Gd-NPs of 1 were uncovered and characterized for the first time. MRI of MDA-MB-468 tumors showed that 1 has enhanced MR contrast within the tumors than that of its scrambled control 1-Scr.

引用

页数：9

共 32 条

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