In Vitro and in Vivo Studies of FePt Nanoparticles for Dual Modal CT/MRI Molecular Imaging

被引:303
作者
Chou, Shang-Wei [3 ]
Shau, Yu-Hong [1 ,2 ]
Wu, Ping-Ching [1 ,2 ,4 ]
Yang, Yu-Sang [1 ,2 ,4 ]
Shieh, Dar-Bin [1 ,2 ,4 ,5 ,6 ]
Chen, Chia-Chun [3 ,7 ]
机构
[1] Natl Cheng Kung Univ, Inst Oral Med, Tainan 70101, Taiwan
[2] Natl Cheng Kung Univ, Dept Stomatol, Tainan 70101, Taiwan
[3] Natl Taiwan Normal Univ, Dept Chem, Taipei 11677, Taiwan
[4] Natl Cheng Kung Univ, Inst Basic Med Sci, Tainan 70101, Taiwan
[5] Natl Cheng Kung Univ, Adv Optoelect Technol Ctr, Tainan 70101, Taiwan
[6] Natl Cheng Kung Univ, Ctr Micro Nano Sci & Technol, Tainan 70101, Taiwan
[7] Acad Sinica, Inst Atom & Mol Sci, Taipei 10617, Taiwan
关键词
IRON-OXIDE NANOPARTICLES; RAY COMPUTED-TOMOGRAPHY; COATED GOLD NANOPARTICLES; MRI CONTRAST AGENTS; MAGNETIC NANOPARTICLES; SIZE; CANCER; NANOCRYSTALS; RELAXIVITY; DELIVERY;
D O I
10.1021/ja1035013
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
The water-solvable FePt nanoparticles of 3, 6, and 12 nm in diameter (3 nm-, 6 nm-, and 12 nm-FePt) were synthesized and applied as a dual modality contrast agent for CT/MRI molecular imaging. These nanoparticles present excellent biocompatibility and hemocompatibility in all test concentrations for the imaging contrast. The biodistribution analysis revealed the highest serum concentration and circulation half-life for 12 nm-FePt, followed by 6 nm-FePt then 3 nm-FePt. Thus, the 3 nm-FePt showed higher brain concentrations. Anti-Her2 antibody conjugated FePt nanoparticles demonstrated molecular expression dependent CT/MRI dual imaging contrast effect in MBT2 cell line and its Her2/neu gene knock out counterpart. Selective contrast enhancement of Her2/neu overexpression cancer lesions in both CT and MRI was found in tumor bearing animal after tail vein injection of the nanoparticles. The 12 nm-FePt outperformed 3 nm-FePt in both imaging modalities. These results indicate the potential of FePt nanoparticles to serve as novel multimodal molecular imaging contrast agents in clinical settings.
引用
收藏
页码:13270 / 13278
页数:9
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