A plant-based expression system for matching cDNA clones and isozymes

被引：5

作者：

Matthews, PR ^{[1
]}

Gubler, F ^{[1
]}

Jacobsen, JV ^{[1
]}

机构：

[1] CSIRO, DIV PLANT IND, CANBERRA, ACT 2601, AUSTRALIA

来源：

PLANT MOLECULAR BIOLOGY REPORTER | 1997年 / 15卷 / 02期

关键词：

Avena sativa; alpha-amylase cDNAs; cDNA isozyme matching; Hordeum vulgare; isoelectric focusing; microparticle bombardment; oat aleurone; transient expression;

D O I：

10.1007/BF02812267

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Isozymes of barley alpha-amylase were matched to cDNAs that encode them using transient expression in oat aleurone layers. Four cDNAs, including two that are previously unpublished, were inserted into oat aleurone cells by microparticle bombardment. The cDNAs were under the control of the Act1 promoter of rice. Expression levels were sufficient for in-gel detection of enzyme activity following isoelectric focusing of aleurone homogenates. The system has also proved useful in characterizing a hybrid beta-glucanase gene.

引用

页码：163 / 169

页数：7

共 23 条

[11]

HUNOLD R, 1994, PLANT J, V5, P593, DOI 10.1046/j.1365-313X.1994.5040593.x

[12] EVIDENCE FOR PRECURSOR FORMS OF THE LOW ISOELECTRIC POINT ALPHA-AMYLASE ISOZYMES SECRETED BY BARLEY ALEURONE CELLS [J].