Soft particle analysis of bacterial cells and its interpretation of cell adhesion behaviors in terms of DLVO theory

被引:99
作者
Hayashi, H
Tsuneda, S
Hirata, A
Sasaki, H
机构
[1] Waseda Univ, Sch Sci & Engn, Dept Chem Engn, Shinjuku Ku, Tokyo 1698555, Japan
[2] Waseda Univ, Dept Environm & Resources Engn, Shinjuku Ku, Tokyo 1698555, Japan
关键词
bacterial adhesion; surface characteristics; electrophoretic mobility; soft particle electrophoresis theory; DLVO theory;
D O I
10.1016/S0927-7765(01)00161-8
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
The electrokinetic properties of two nitrifying strains, Nitrosomonas europaea and Nitrobacter winogradskyi, and three heterotrophic bacteria, Escherichia coli, Pseudomonas putida and Pseudomonas aeruginosa, were examined by electrophoretic mobility measurement and analyzed using the soft particle electrophoresis theory that is suitable for biological particles, The bacterial adhesion characteristics onto glass bead substratum were also evaluated by packed bed method. The mobility of the bacterial cells employed converged to a non-zero value as the ionic concentration increased, suggesting that the bacterial cells exhibited typical soft particle characteristics. Moreover, cell surface potentials based on the soft particle theory were lower than those estimated by the conventional Smoluchowski formula, i.e. zeta potential. Cell collision efficiencies onto glass beads (alpha(0)) were largely dependent on interfacial interaction, although almost electrically neutral P. aeruginosa did not follow that trend. From a comparison of alpha(0) with DLVO interaction energy maximum (V-max), it was assumed that heterocoagulation between cell and substratum at primary minimum potential took place under V-max of 24-34 kT based on soft particle analysis. On the other hand, V-max predictions using the Smoluchowski theory gave 81-223 kT, which indicated the possibility of overestimating electrostatic repulsive forces by the conventional Smoluchowski theory. Thus, the application of this new electrophoresis theory to several kinds of bacterial cells has led to the revision of the interpretation of bacterial mobility data and provided a more detailed understanding of the bacterial adhesion phenomenon. (C) 2001 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:149 / 157
页数:9
相关论文
共 25 条
[1]   SURFACE THERMODYNAMICS OF BACTERIAL ADHESION [J].
ABSOLOM, DR ;
LAMBERTI, FV ;
POLICOVA, Z ;
ZINGG, W ;
VANOSS, CJ ;
NEUMANN, AW .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1983, 46 (01) :90-97
[2]   'Soft-particle' analysis of the electrophoretic mobility of a fibrillated and non-fibrillated oral streptococcal strain:: Streptococcus salivarius [J].
Bos, R ;
van der Mei, HC ;
Busscher, HJ .
BIOPHYSICAL CHEMISTRY, 1998, 74 (03) :251-255
[3]  
BRYERS JD, 1994, COLLOID SURFACE B, V2, P9, DOI 10.1016/0927-7765(94)80013-8
[4]   THE EFFECTS OF PH, IONIC-STRENGTH AND ORGANIC-PHASE ON THE BACTERIAL ADHESION TO HYDROCARBONS (BATH) TEST [J].
BUNT, CR ;
JONES, DS ;
TUCKER, IG .
INTERNATIONAL JOURNAL OF PHARMACEUTICS, 1993, 99 (2-3) :93-98
[5]   MEASUREMENT OF THE SURFACE FREE-ENERGY OF BACTERIAL-CELL SURFACES AND ITS RELEVANCE FOR ADHESION [J].
BUSSCHER, HJ ;
WEERKAMP, AH ;
VANDERMEI, HC ;
VANPELT, AWJ ;
DEJONG, HP ;
ARENDS, J .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1984, 48 (05) :980-983
[6]  
HAYASHI H, IN PRESS STUDIES SUR
[7]   The DLVO theory in microbial adhesion [J].
Hermansson, M .
COLLOIDS AND SURFACES B-BIOINTERFACES, 1999, 14 (1-4) :105-119
[8]   MUTUAL COAGULATION OF COLLOIDAL DISPERSIONS [J].
HOGG, R ;
HEALY, TW ;
FUERSTENAU, DW .
TRANSACTIONS OF THE FARADAY SOCIETY, 1966, 62 (522P) :1638-+
[9]  
HUNTER RJ, 1985, FDN COLLOID SCI, V1
[10]   Adhesion of the positively charged bacterium Stenotrophomonas (Xanthomonas) maltophilia 70401 to glass and teflon [J].
Jucker, BA ;
Harms, H ;
Zehnder, AJB .
JOURNAL OF BACTERIOLOGY, 1996, 178 (18) :5472-5479