Effects of 28-homobrassinolide on growth, lipid peroxidation and antioxidative enzyme activities in seedlings of Zea mays L. under salinity stress

被引：67

作者：

Arora, Nitika ^{[1
]}

Bhardwaj, Renu ^{[1
]}

Sharma, Priyanka ^{[1
]}

Arora, Hardesh Kumar ^{[1
]}

机构：

[1] Guru Nanak Dev Univ, Dept Bot & Environm Sci, Amritsar 143005, Punjab, India

来源：

ACTA PHYSIOLOGIAE PLANTARUM | 2008年 / 30卷 / 06期

关键词：

Antioxidative enzymes; 28-Homobrassinolide; Lipid peroxidation; Maize; NaCl;

D O I：

10.1007/s11738-008-0188-9

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The effects of 28-homobrassinolide (28-homoBL) on seedling growth, lipid peroxidation and antioxidative enzyme activities in the seedlings of Zea mays L. (var. Partap-1) under salt (NaCl) stress were studied. The surface-sterilized seeds were germinated in petriplates containing different concentrations of NaCl (25, 50, 75 and 100 mM) only, 28-homoBL (10(-7), 10(-9) and 10(-11) M) only and NaCl supplemented with 28-homoBL for 7 days. The activities of superoxide dismutase (SOD, EC 1.15.1.1), guaiacol peroxidase (POD, EC 1.11.1.7), catalase (CAT, EC 1.11.1.6), ascorbate peroxidase (APOX, EC 1.11.1.11) and glutathione reductase (EC 1.6.4.2) were analysed in 7 day-old seedlings. It was observed that 28-homoBL treatments reduced the toxicity of salt on seedling growth considerably. Lipid peroxidation level was significantly increased under saline stress, but lowered with 28-homoBL applications revealing less oxidative damage. Further 28-homoBL treatments to the seedlings showed an enhancement in activities of SOD, POD, CAT and APOX. The activities of all antioxidative enzymes were further increased in seedlings treated with solution containing 28-homoBL and salt together as compared to seedlings treated with different concentration of salt solution only.

引用

页码：833 / 839

页数：7

共 43 条

[1]

AEBI H, 1983, METHOD ENZYMAT AN, V2, P673

[2]

[Anonymous], 2006, PLANT MOL PHYSL CURR

[3] Application of brassinosteroids to rice seeds (Oryza sativa L.) reduced the impact of salt stress on growth, prevented photosynthetic pigment loss and increased nitrate reductase activity [J].

Anuradha, S ;

Rao, SSR .

PLANT GROWTH REGULATION, 2003, 40 (01) :29-32

[4] Effect of brassinosteroids on salinity stress induced inhibition of seed germination and seedling growth of rice (Oryza sativa L.) [J].

Anuradha, S ;

Rao, SSS .

PLANT GROWTH REGULATION, 2001, 33 (02) :151-153

[5]

Arora A, 2002, CURR SCI INDIA, V82, P1227

[6]

Asada K., 1987, Photoinhibition, P227

[7] The chemical characteristic and distribution of brassinosteroids in plants [J].

Bajguz, A ;

Tretyn, A .

PHYTOCHEMISTRY, 2003, 62 (07) :1027-1046

[8]

Barket Ali, 2005, General and Applied Plant Physiology, V31, P55

[9]

Bhardwaj Renu, 2007, Asian Journal of Plant Sciences, V6, P765

[10] Loss-of-function mutations in DET2 gene lead to an enhanced resistance to oxidative stress in Arabidopsis [J].

Cao, SQ ;

Xu, QT ;

Cao, YJ ;

Qian, K ;

An, K ;

Zhu, Y ;

Hu, BZ ;

Zhao, HF ;

Kuai, BK .

PHYSIOLOGIA PLANTARUM, 2005, 123 (01) :57-66

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