The development of a combined surface adhesion and polymerase chain reaction technique in the rapid detection of Listeria monocytogenes in meat and poultry

被引:22
作者
Duffy, G
Cloak, OM [1 ]
Sheridan, JJ
Blair, IS
McDowell, DA
机构
[1] TEAGASC, Natl Food Ctr, Dublin 15, Ireland
[2] Univ Ulster, Newtownabbey BT37 0QB, Antrim, North Ireland
关键词
surface adhesion; Listeria; PCR;
D O I
10.1016/S0168-1605(99)00091-4
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
A procedure combining enrichment surface adhesion and polymerase chain detection (SA-PCR) was developed and applied in the detection of Listeria monocytogenes in meat products. Minced beef samples were inoculated with L. monocytogens (log(10)3 cfu g(-1)) and incubated for 10 h at 30 degrees C in buffered peptone water. L. monocytogenes was recovered from the culture by attachment to a polycarbonate membrane immersed for 15 min in the enriched meat culture. The membrane and attached bacteria were removed from the culture and the membrane dissolved in phenol:chloroform. The DNA was extracted from the bacteria and a PCR assay was carried out using primers directed against the listerolysin O gene of L. monocytogenes. The combined (SA-PCR) technique had a detection limit of log(10) 4.0 cfu ml(-1) in enriched meat cultures. The rapid technique was applied to a small number of retail samples (n = 100) acid was found to compare favourably to the standard cultural method, A total of 12 samples were confirmed positive for L. monocytogenes using the standard cultural method and the SA-PCR assay. No false positives or negatives were recorded by either method. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:151 / 159
页数:9
相关论文
共 50 条
[21]  
HOFFMAN J, 1987, GENE, V547, P267
[22]   Prevalence of Listeria spp in meat and poultry products [J].
Jay, JM .
FOOD CONTROL, 1996, 7 (4-5) :209-214
[23]  
KACZMARSKI EB, 1989, LANCET, V1, P549
[24]   INCIDENCE OF LISTERIA SPP AND LISTERIA-MONOCYTOGENES IN A POULTRY-PROCESSING ENVIRONMENT AND IN POULTRY PRODUCTS AND THEIR RAPID CONFIRMATION BY MULTIPLEX PCR [J].
LAWRENCE, LM ;
GILMOUR, A .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1994, 60 (12) :4600-4604
[25]   EPIDEMIC LISTERIOSIS ASSOCIATED WITH MEXICAN-STYLE CHEESE [J].
LINNAN, MJ ;
MASCOLA, L ;
LOU, XD ;
GOULET, V ;
MAY, S ;
SALMINEN, C ;
HIRD, DW ;
YONEKURA, ML ;
HAYES, P ;
WEAVER, R ;
AUDURIER, A ;
PLIKAYTIS, BD ;
FANNIN, SL ;
KLEKS, A ;
BROOME, CV .
NEW ENGLAND JOURNAL OF MEDICINE, 1988, 319 (13) :823-828
[26]  
LORBER B, 1990, TOPICS IND MICROBIOL, P44
[27]  
MCCLAIN D, 1987, USDA FSIS MICROBIOLO
[28]   The relationship between Listeria and listeriosis [J].
McLauchlin, J .
FOOD CONTROL, 1996, 7 (4-5) :187-193
[29]   HUMAN LISTERIOSIS AND PATE - A POSSIBLE ASSOCIATION [J].
MCLAUCHLIN, J ;
HALL, SM ;
VELANI, SK ;
GILBERT, RJ .
BRITISH MEDICAL JOURNAL, 1991, 303 (6805) :773-775
[30]   EXPRESSION IN ESCHERICHIA-COLI AND SEQUENCE-ANALYSIS OF THE LISTERIOLYSIN-O DETERMINANT OF LISTERIA-MONOCYTOGENES [J].
MENGAUD, J ;
VICENTE, MF ;
CHENEVERT, J ;
PEREIRA, JM ;
GEOFFROY, C ;
GICQUELSANZEY, B ;
BAQUERO, F ;
PEREZDIAZ, JC ;
COSSART, P .
INFECTION AND IMMUNITY, 1988, 56 (04) :766-772