Cloning, characterization, and sequence analysis of the clcE gene encoding the maleylacetate reductase of Pseudomonas sp. strain B13

被引：24

作者：

Kasberg, T ^{[1
]}

Seibert, V ^{[1
]}

Schlomann, M ^{[1
]}

Reineke, W ^{[1
]}

机构：

[1] BERG UNIV GESAMTHSCH WUPPERTAL,D-42097 WUPPERTAL,GERMANY

来源：

JOURNAL OF BACTERIOLOGY | 1997年 / 179卷 / 11期

关键词：

D O I：

10.1128/jb.179.11.3801-3803.1997

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A 3,167-bp PstI fragment of genomic DNA from Pseudomonas sp. strain B13 nas cloned and sequenced. The gene clcE consists of 1,059 nucleotides encoding a protein of 352 amino acids with a calculated mass of 37,769 Da which showed maleylacetate reductase activity. The protein had significant sequence similarities with the polypeptides encoded by tcbF of pP51 (59.4% identical positions), tfdF of pJP4 (55.1%), and tftE of Burkholderia cepacia AC1100 (53.1%). The function of TcbF as maleylacetate reductase was established by an enzyme assay.

引用

页码：3801 / 3803

页数：3

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