GRecon: A Method for the Lipid Reconstitution of Membrane Proteins

被引：18

作者：

Althoff, Thorsten ^{[1
]}

Davies, Karen M. ^{[1
]}

Schulze, Sabrina ^{[1
]}

Joos, Friederike ^{[1
]}

Kuehlbrandt, Werner ^{[1
]}

机构：

[1] Max Planck Inst Biophys, D-60438 Frankfurt, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2012年 / 51卷 / 33期

关键词：

cyclodextrins; detergents; liposomes; membrane proteins; structural biology; STRUCTURAL BASIS; COMPLEX;

D O I：

10.1002/anie.201202094

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Density gradients with increasing concentrations of cyclodextrin and detergent-destabilized liposomes are loaded with detergent-solubilized membrane proteins in a new approach for reconstituting membrane proteins. During centrifugation, the detergent is gradually replaced by the lipid. This gentle method separates proteoliposomes from empty liposomes and is particularly suitable for the lipid reconstitution of large, fragile membrane proteins. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：8343 / 8347

页数：5

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