Relaxation of transcription-induced negative supercoiling is an essential function of Escherichia coli DNA topoisomerase I

被引：60

作者：

Massé, E ^{[1
]}

Drolet, M ^{[1
]}

机构：

[1] Univ Montreal, Dept Microbiol & Immunol, Montreal, PQ H3C 3J7, Canada

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 1999年 / 274卷 / 23期

关键词：

D O I：

10.1074/jbc.274.23.16654

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

It has been suggested that the essential function of DNA topoisomerase I in Escherichia coli is to prevent chromosomal DNA from reaching an unacceptably high level of global negative supercoliing. However, other in vivo studies have shown that DNA topoisomerase I is very effective in removing local negative supercoliing generated during transcription elongation. To determine whether topoisomerase I is essential for controlling global or local DNA supercoliing, we have prepared a set of topA null mutant strains in combination with different plasmid DNAs. Although we found a correlation between the severity of the growth defect with both transcription-induced and global supercoliing, near to complete growth inhibition correlated only with transcription-induced supercoliing. This result strongly suggests that the major function of DNA topoisomerase I is to relax local negative supercoliing generated during transcription elongation.

引用

页码：16654 / 16658

页数：5

共 17 条

[1] DYNAMICS OF DNA SUPERCOILING BY TRANSCRIPTION IN ESCHERICHIA-COLI [J].

COOK, DN ;

MA, D ;

PON, NG ;

HEARST, JE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (22) :10603-10607

[2] ESCHERICHIA-COLI DNA TOPOISOMERASE-I MUTANTS HAVE COMPENSATORY MUTATIONS IN DNA GYRASE GENES [J].

DINARDO, S ;

VOELKEL, KA ;

STERNGLANZ, R ;

REYNOLDS, AE ;

WRIGHT, A .

CELL, 1982, 31 (01) :43-51

[3] CONTROL OF BACTERIAL-DNA SUPERCOILING [J].

DRLICA, K .

MOLECULAR MICROBIOLOGY, 1992, 6 (04) :425-433

[4] OVEREXPRESSION OF RNASE-H PARTIALLY COMPLEMENTS THE GROWTH DEFECT OF AN ESCHERICHIA-COLI DELTA-TOPA MUTANT - R-LOOP FORMATION IS A MAJOR PROBLEM IN THE ABSENCE OF DNA TOPOISOMERASE-I [J].

DROLET, M ;

PHOENIX, P ;

MENZEL, R ;

MASSE, E ;

LIU, LF ;

CROUCH, RJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (08) :3526-3530

[5]

DROLET M, 1994, J BIOL CHEM, V269, P2068

[6] SUPERCOILING OF THE DNA-TEMPLATE DURING TRANSCRIPTION [J].

LIU, LF ;

WANG, JC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (20) :7024-7027

[7] ANCHORING OF DNA TO THE BACTERIAL CYTOPLASMIC MEMBRANE THROUGH COTRANSCRIPTIONAL SYNTHESIS OF POLYPEPTIDES ENCODING MEMBRANE-PROTEINS OR PROTEINS FOR EXPORT - A MECHANISM OF PLASMID HYPERNEGATIVE SUPERCOILING IN MUTANTS DEFICIENT IN DNA TOPOISOMERASE-I [J].

LYNCH, AS ;

WANG, JC .

JOURNAL OF BACTERIOLOGY, 1993, 175 (06) :1645-1655

[8] Escherichia coli DNA topoisomerase I inhibits R-loop formation by relaxing transcription-induced negative supercoiling [J].

Massé, E ;

Drolet, M .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (23) :16659-16664

[9]

Miller J.H., 1992, SHORT COURSE BACTERI, V1

[10] Roles of DNA topoisomerases in the regulation of R-loop formation in vitro [J].

Phoenix, P ;

Raymond, MA ;

Masse, E ;

Drolet, M .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (03) :1473-1479

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