Administration of recombinant rhesus interleukin-12 during acute simian immunodeficiency virus (SIV) infection leads to decreased viral loads associated with prolonged survival in SIVmac251-infected rhesus macaques

被引:42
作者
Ansari, AA
Mayne, AE
Sundstrom, JB
Bostik, P
Grimm, B
Altman, JD
Villinger, F
机构
[1] Emory Univ, Sch Med, Winship Canc Inst, Dept Pathol & Lab Med, Atlanta, GA 30322 USA
[2] Emory Univ, Sch Med, Dept Microbiol, Atlanta, GA 30322 USA
关键词
D O I
10.1128/JVI.76.4.1731-1743.2002
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The ability of recombinant rhesus interleukin-12 (rMamu-IL-12) administration during acute simian immunodeficiency virus SIVmac251 infection to influence the quality of the antiviral immune responses was assessed in rhesus macaques. Group I (n = 4) was the virus-only control group. Group 11 and III received a conditioning regimen of rMamu-IL-12 (10 and 20 mug/kg, respectively, subcutaneously [s.c.]) on days -2 and -0. Thereafter, group II received 2 mug of IL-12 per kg and group III received 10 mug/kg s.c. twice a week for 8 weeks. On day 0 all animals were infected with SIVmac251 intravenously. While all four group I animals and three of four group II animals died by 8 and 10 months post infection (p.i.), all four group III animals remained alive for >20 months p.i. The higher IL-12 dose led to lower plasma viral loads and markedly lower peripheral blood mononuclear cell and lymph node proviral DNA loads. During the acute viremia phase, the high-IL12-dose monkeys showed an increase in CD3(-) CD8alpha/alpha(+) and CD3(+) CD8alpha/alpha(+) cells and, unlike the control and low-IL-12-dose animals, did not demonstrate an increase in CD4(+) CD45RA(+) CD62L(+) naive cells. The high-IL-12-dose animals also demonstrated that both CD8alpha/beta(+) and CD8alpha/beta(+) cells produced antiviral factors early p.i., whereas only CD8alpha/beta(+) cells retained this function late p.i. Long-term survival correlated with sustained high levels of SIV gag/pol and SIV env cytotoxic T lymphocytes and retention of high memory responses against nominal antigens. This is the first study to demonstrate the capacity of IL-12 to significantly protect macaques from SIV-induced disease, and it provides a useful model to more precisely identify correlates of virus-specific disease-protective responses.
引用
收藏
页码:1731 / 1743
页数:13
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