Iron oxide nanoparticles-chitosan composite based glucose biosensor

被引：372

作者：

Kaushika, Ajeet ^{[1
,2
]}

Khan, Raju ^{[1
]}

Solanki, Pratima R. ^{[1
]}

Pandey, Pratibha ^{[1
]}

Alam, Javed ^{[2
]}

Ahmad, Sharif ^{[2
]}

Malhotra, B. D. ^{[1
]}

机构：

[1] Natl Phys Lab, Biomol Elect & Conducting Polymer Res Grp, New Delhi 110012, India

[2] Jamia Millia Islamia, Dept Chem, Mat Res Lab, New Delhi 110025, India

来源：

BIOSENSORS & BIOELECTRONICS | 2008年 / 24卷 / 04期

关键词：

Biosensor; Chitosan; Fe3O4; Nanoparticle; Nanocomposite; Glucose oxidase;

D O I：

10.1016/j.bios.2008.06.032

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Iron oxide (Fe3O4) nanoparticles prepared using co-precipitation method have been dispersed in chitosan (CH) solution to fabricate nanocomposite film on indium-tin oxide (ITO) glass plate. Glucose oxidase (GOx) has been immobilized onto this CH-Fe3O4 nanocomposite film via physical adsorption. The size of the Fe3O4 nanoparticles estimated using X-ray diffraction (XRD) pattern and transmission electron microscopy (TEM) has been found to be similar to 22 nm. The CH-Fe3O4 nanocomposite film and GOx/CH-Fe3O4/ITO bioelectrode have been characterized using UV-visible and Fourier transform infrared (FTIR) spectroscopic and scanning electron microscopy (SEM) techniques, respectively. This GOx/CH-Fe3O4/ITO nanocomposite bioelectrode has response time of 5 s, linearity as 10-400 mg dL(-1) of glucose, sensitivity as 9.3 mu A/(mg dL cm(2)) and shelf life of about 8 weeks under refrigerated conditions. The value of Michaelis-Menten (Km)constant obtained as 0.141 mM indicates high affinity of immobilized GOx towards the substrate (glucose). (C) 2008 Published by Elsevier B.V.

引用

页码：676 / 683

页数：8

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