High-throughput screening for biocatalysts

被引:123
作者
Wahler, D [1 ]
Reymond, JL [1 ]
机构
[1] Univ Bern, Dept Chem & Biochem, CH-3012 Bern, Switzerland
关键词
D O I
10.1016/S0958-1669(01)00260-9
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Recent progress in high-throughput enzyme assays includes new examples of fluorogenic and chromogenic substrates, fluorescence resonance energy transfer substrates, and applications of the pH and pM indicator methods. Recent developments of Horeau's pseudo-enantiomer derivatisation method to screen enantioselectivities in high-throughput have also been reported.
引用
收藏
页码:535 / 544
页数:10
相关论文
共 54 条
[31]   Reaction microarrays: A method for rapidly determining the enantiomeric excess of thousands of samples [J].
Korbel, GA ;
Lalic, G ;
Shair, MD .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2001, 123 (02) :361-362
[32]   NOVEL FLUOROGENIC SUBSTRATES FOR ASSAYING RETROVIRAL PROTEASES BY RESONANCE ENERGY-TRANSFER [J].
MATAYOSHI, ED ;
WANG, GT ;
KRAFFT, GA ;
ERICKSON, J .
SCIENCE, 1990, 247 (4945) :954-958
[33]   Directed evolution of new glycosynthases from Agrobacterium β-glucosidase:: a general screen to detect enzymes for oligosaccharide synthesis [J].
Mayer, C ;
Jakeman, DL ;
Mah, M ;
Karjala, G ;
Gal, L ;
Warren, RAJ ;
Withers, SG .
CHEMISTRY & BIOLOGY, 2001, 8 (05) :437-443
[34]  
Meyer J, 2000, ANGEW CHEM INT EDIT, V39, P1453, DOI 10.1002/(SICI)1521-3773(20000417)39:8<1453::AID-ANIE1453>3.0.CO
[35]  
2-7
[36]   High-throughput screening of enzyme libraries [J].
Olsen, M ;
Iverson, B ;
Georgiou, G .
CURRENT OPINION IN BIOTECHNOLOGY, 2000, 11 (04) :331-337
[37]   Function-based isolation of novel enzymes from a large library [J].
Olsen, MJ ;
Stephens, D ;
Griffiths, D ;
Daugherty, P ;
Georgiou, G ;
Iverson, BL .
NATURE BIOTECHNOLOGY, 2000, 18 (10) :1071-1074
[38]  
PEARSON B, 1963, LAB INVEST, V12, P1249
[39]  
Reetz MT, 2001, ANGEW CHEM INT EDIT, V40, P284, DOI 10.1002/1521-3773(20010119)40:2<284::AID-ANIE284>3.0.CO
[40]  
2-N