In vitro self-assembled HCV core virus-like particles induce a strong antibody immune response in sheep

被引：18

作者：

Acosta-Rivero, N

Alvarez-Obregón, JC

Musacchio, A

Falcón, V

Dueñas-Carrera, S

Marante, J

Menéndez, I

Morales, J

机构：

[1] Ctr Genet Engn & Biotechnol, Electron Microscopy Lab, Div Vaccines, Havana 10600, Cuba

[2] Ctr Genet Engn & Biotechnol, Electron Microscopy Lab, Dept Phys Chem, Havana 10600, Cuba

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2002年 / 290卷 / 01期

关键词：

hepatitis C; core antigen; virus-like particles; P; pastoris;

D O I：

10.1006/bbrc.2001.6177

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The in vitro self-assembly properties of the entire hepatitis C virus core protein (HCcAg) obtained from Pichia pastoris cells and the induction of specific antibody immune response were studied. HCcAg was purified as a low-molecular-weight species by electroelution under denaturing conditions for confirmation of its self-assembly properties. After renaturalization, electron microscopy showed that HCcAg assembled into spherical particles of 30 nm. HCcAg also showed homogeneity and was specifically recognized by serum from a chronic HCV carrier patient. The data indicated that in vitro assembly of HCcAg, into virus-like particles resembling HCV nucleocapsid particles at a mature stage, is an intrinsic quality of this protein. Finally, HCcAg generated a strong antibody immune response in sheep, suggesting its usefulness for stimulating the host immune response against HCV. (C) 2002 Elsevier Science.

引用

页码：300 / 304

页数：5

共 30 条

[1] Characterization of the HCV core virus-like particles produced in the methylotrophic yeast Pichia pastoris [J].