In vitro self-assembled HCV core virus-like particles induce a strong antibody immune response in sheep

被引：18

作者：

Acosta-Rivero, N

Alvarez-Obregón, JC

Musacchio, A

Falcón, V

Dueñas-Carrera, S

Marante, J

Menéndez, I

Morales, J

机构：

[1] Ctr Genet Engn & Biotechnol, Electron Microscopy Lab, Div Vaccines, Havana 10600, Cuba

[2] Ctr Genet Engn & Biotechnol, Electron Microscopy Lab, Dept Phys Chem, Havana 10600, Cuba

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2002年 / 290卷 / 01期

关键词：

hepatitis C; core antigen; virus-like particles; P; pastoris;

D O I：

10.1006/bbrc.2001.6177

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The in vitro self-assembly properties of the entire hepatitis C virus core protein (HCcAg) obtained from Pichia pastoris cells and the induction of specific antibody immune response were studied. HCcAg was purified as a low-molecular-weight species by electroelution under denaturing conditions for confirmation of its self-assembly properties. After renaturalization, electron microscopy showed that HCcAg assembled into spherical particles of 30 nm. HCcAg also showed homogeneity and was specifically recognized by serum from a chronic HCV carrier patient. The data indicated that in vitro assembly of HCcAg, into virus-like particles resembling HCV nucleocapsid particles at a mature stage, is an intrinsic quality of this protein. Finally, HCcAg generated a strong antibody immune response in sheep, suggesting its usefulness for stimulating the host immune response against HCV. (C) 2002 Elsevier Science.

引用

收藏

页码：300 / 304

页数：5

相关论文

共 30 条

[21] DNA encapsidation by viruslike particles assembled in insect cells from the major capsid protein VP1 of B-lymphotropic papovavirus [J].

Pawlita, M ;

Muller, M ;

Oppenlander, M ;

Zentgraf, H ;

Herrmann, M .

JOURNAL OF VIROLOGY, 1996, 70 (11) :7517-7526

[22] Characterization of hepatitis C virus core-specific immune responses primed in rhesus macaques by a nonclassical ISCOM vaccine [J].

Polakos, NK ;

Drane, D ;

Cox, J ;

Ng, P ;

Selby, MJ ;

Chien, D ;

O'Hagan, DT ;

Houghton, M ;

Paliard, X .

JOURNAL OF IMMUNOLOGY, 2001, 166 (05) :3589-3598

[23] BIOSYNTHESIS AND BIOCHEMICAL-PROPERTIES OF THE HEPATITIS-C VIRUS CORE PROTEIN [J].

SANTOLINI, E ;

MIGLIACCIO, G ;

LAMONICA, N .

JOURNAL OF VIROLOGY, 1994, 68 (06) :3631-3641

[24] Recombinant parvovirus-like particles as an antigen carrier: A novel nonreplicative exogenous antigen to elicit protective antiviral cytotoxic T cells [J].

Sedlik, C ;

Saron, MF ;

Sarraseca, J ;

Casal, I ;

Leclerc, C .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (14) :7503-7508

[25] AN EPITOPE IN HEPATITIS-C VIRUS CORE REGION RECOGNIZED BY CYTOTOXIC T-CELLS IN MICE AND HUMANS [J].

SHIRAI, M ;

OKADA, H ;

NISHIOKA, M ;

AKATSUKA, T ;

WYCHOWSKI, C ;

HOUGHTEN, R ;

PENDLETON, CD ;

FEINSTONE, SM ;

BERZOFSKY, JA .

JOURNAL OF VIROLOGY, 1994, 68 (05) :3334-3342

[26] Priming of cytotoxic T lymphocytes by five heat-aggregated antigens in vivo: conditions, efficiency, and relation to antibody responses [J].

Speidel, K ;

Osen, W ;

Faath, S ;

Hilgert, I ;

Obst, R ;

Braspenning, J ;

Momburg, F ;

Hammerling, GJ ;

Rammensee, HG .

EUROPEAN JOURNAL OF IMMUNOLOGY, 1997, 27 (09) :2391-2399

[27] P26-PROTEIN AND 33-NM PARTICLE ASSOCIATED WITH NUCLEOCAPSID OF HEPATITIS-C VIRUS RECOVERED FROM THE CIRCULATION OF INFECTED HOSTS [J].

TAKAHASHI, K ;

KISHIMOTO, S ;

YOSHIZAWA, H ;

OKAMOTO, H ;

YOSHIKAWA, A ;

MISHIRO, S .

VIROLOGY, 1992, 191 (01) :431-434

[28] INTERNAL RIBOSOME ENTRY SITE WITHIN HEPATITIS-C VIRUS-RNA [J].

TSUKIYAMAKOHARA, K ;

IIZUKA, N ;

KOHARA, M ;

NOMOTO, A .

JOURNAL OF VIROLOGY, 1992, 66 (03) :1476-1483

[29]

WEIGNER AJ, 1990, LANCET, V336, P695

[30] The native form and maturation process of hepatitis C virus core protein [J].

Yasui, K ;

Wakita, T ;

Tsukiyama-Kohara, K ;

Funahashi, S ;

Ichikawa, M ;

Kajita, T ;

Moradpour, D ;

Wands, JR ;

Kohara, M .

JOURNAL OF VIROLOGY, 1998, 72 (07) :6048-6055