A cloth strip bioreactor with immobilized glucoamylase

被引：26

作者：

D'Souza, SF ^{[1
]}

Kubal, BS ^{[1
]}

机构：

[1] Bhabha Atom Res Ctr, Nucl Agr & Biotechnol Div, Mumbai 4000085, India

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 2002年 / 51卷 / 02期

关键词：

adsorption; bioreactor; cotton cloth; cross-linking; immobilization; polyethyleneimine;

D O I：

10.1016/S0165-022X(02)00008-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Glucoamylase was immobilized on polyethylenimine (PEI)-coated cotton cloth by adsorption followed by cross-linking with 0.2% glutaraldehyde in the presence of starch. Optimal adsorption of the enzyme was seen when cloth treated with 2% PEI was contacted with the enzyme for 50 min. pH and temperature optima profiles were not changed appreciably on immobilization. However, the bound enzyme exhibited a higher thermal stability. The enzyme-bound cloth strips were used in a specially designed bioreactor for the continuous hydrolysis of starch. The reactor could be operated for over 21 days retaining about 70% of the original activity. An operational temperature of 45degreesC was found to be optimal. (C) 2002 Elsevier Science B.V All rights reserved.

引用

页码：151 / 159

页数：9

共 20 条

[1] Characterization of glucoamylase immobilized on magnetic poly(styrene) particles [J].

Bahar, T ;

Çelebi, SS .

ENZYME AND MICROBIAL TECHNOLOGY, 1998, 23 (05) :301-304

[2] POLYETHYLENEIMINE IN IMMOBILIZATION OF BIOCATALYSTS [J].

BAHULEKAR, R ;

AYYANGAR, NR ;

PONRATHNAM, S .

ENZYME AND MICROBIAL TECHNOLOGY, 1991, 13 (11) :858-868

[3]

Bickerstaff G, 1997, METHODS BIOTECHNOLOG, V1

[4]

D'Souza SF, 1999, CURR SCI INDIA, V77, P69

[5] Immobilization of bakers yeast on jute fabric through adhesion using polyethylenimine: application in an annular column reactor for the inversion of sucrose [J].

D'Souza, SF ;

Melo, JS .

PROCESS BIOCHEMISTRY, 2001, 36 (07) :677-681

[6]

DSOUZA SF, 1988, APPL MICROBIOL BIOT, V29, P136

[7] IMMOBILIZATION OF YEAST-CELLS BY ADHESION TO GLASS-SURFACE USING POLYETHYLENIMINE [J].

DSOUZA, SF ;

MELO, JS ;

DESHPANDE, A ;

NADKARNI, GB .

BIOTECHNOLOGY LETTERS, 1986, 8 (09) :643-648

[8]

DSOUZA SF, 1990, FOOD BIOTECHNOL, V4, P373

[9] HYDROLYSIS OF CONCENTRATED SUCROSE SYRUPS BY INVERTASE IMMOBILIZED ON ANION-EXCHANGER WASTE COTTON THREAD [J].

GODBOLE, SS ;

KUBAL, BS ;

DSOUZA, SF .

ENZYME AND MICROBIAL TECHNOLOGY, 1990, 12 (03) :214-217

[10] FILM FERMENTER FOR ETHANOL-PRODUCTION BY YEAST IMMOBILIZED ON COTTON CLOTH [J].

JOSHI, S ;

YAMAZAKI, H .

BIOTECHNOLOGY LETTERS, 1984, 6 (12) :797-802

← 1 2 →