The GapA/B gene duplication marks the origin of streptophyta (Charophytes and land plants)

被引:61
作者
Petersen, Joern
Teich, Rene
Becker, Burkhard
Cerff, Ruediger
Brinkmann, Henner
机构
[1] Tech Univ Carolo Wilhelmina Braunschweig, Inst Genet, Braunschweig, Germany
[2] Univ Cologne, Inst Bot, D-5000 Cologne, Germany
[3] Univ Montreal, Dept Biochim, Montreal, PQ H3C 3J7, Canada
关键词
gene duplication; land plant origin; glyceraldehyde-3-phosphate dehydrogenase; CP12; Mesostigma viride; Cyanophora paradoxa;
D O I
10.1093/molbev/msj123
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Independent evidence from morphological, ultrastructural, biochemical, and molecular data have shown that land plants originated from charophycean green algae. However, the branching order within charophytes is still unresolved, and contradictory phylogenies about, for example,the position of the unicellular green alga Mesostigma viride are difficult to reconcile. A comparison of nuclear-encoded Calvin cycle glyceraldehyde-3-phosphate dehydrogenases (GAPDH) indicates that a crucial duplication of the GapA gene occurred early in land plant evolution. The duplicate called GapB acquired a characteristic carboxy-terminal extension (CTE) from the general regulator of the Calvin cycle CP12. This CTE is responsible for thioredoxin-dependent light/dark regulation. In this work, we established GapA, GapB, and CP12 sequences from bryophytes, all orders of charophyte as well as chlorophyte green algae, and the glaucophyte Cyanophora paradoxa. Comprehensive phylogenetic analyses of all available plastid GAPDH sequences suggest that glaucophytes and green plants are sister lineages and support a positioning of Mesostigma basal to all charophycean algae. The exclusive presence of GapB in terrestrial plants, charophytes, and Mesostigma dates the GapA/B gene duplication to the common ancestor of Streptophyta. The conspicuously high degree of GapB sequence conservation suggests an important metabolic role of the newly gained regulatory function. Because the GapB-mediated protein aggregation most likely ensures the complete blockage of the Calvin cycle at night, we propose that this mechanism is also crucial for efficient starch mobilization. This innovation may be one prerequisite for the development of storage tissues in land plants.
引用
收藏
页码:1109 / 1118
页数:10
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