Magnetic resonance tracking of nanoparticle labelled neural stem cells in a rat's spinal cord

被引：32

作者：

Wang, F. H. ^{[1
]}

Lee, I. H.

Holmstrom, N.

Yoshitake, T.

Kim, D. K.

Muhammed, M.

Frisen, J.

Olson, L.

Spenger, C.

Kehr, J.

机构：

[1] Karolinska Inst, Dept Neurosci, S-17177 Stockholm, Sweden

[2] Karolinska Inst, Dept Cell & Mol Biol, S-17177 Stockholm, Sweden

[3] Karolinska Inst, Dept Physiol & Pharmacol, S-17177 Stockholm, Sweden

[4] Royal Inst Technol, Mat Chem Div, S-10044 Stockholm, Sweden

来源：

NANOTECHNOLOGY | 2006年 / 17卷 / 08期

关键词：

D O I：

10.1088/0957-4484/17/8/018

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

Neural stem cells isolated from an adult rat's spinal cord were loaded with superparamagnetic gold-coated monocrystalline iron oxide nanoparticles (Au-MION) intended for use as contrast enhancers in magnetic resonance imaging (MRI). A dose-dependent attenuation of MRI signals was observed for Au-MION down to 0.001 mu gFe/mu l and for nanoparticle-loaded clusters of only 20 cells. The labelled cells were infused into the spinal cord of anaesthetized rats and tracked by MRI at 1 h, 48 h and 1 month post-injection. Histological analysis revealed that MRI signals correlated well with gold-positive staining of transplanted cells. The present results show that Au-MION exerts powerful contrast-enhancing properties and may represent novel MRI labels for labelling and tracking the transplanted cells in vivo.

引用

页码：1911 / 1915

页数：5

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