Functional study of immature dendritic cells co-transfected with IL-10 and TGF-beta 1 genes in vitro

被引：34

作者：

Chen, Lihong ^{[1
]}

Qiu, Minglian ^{[2
]}

He, Wubing ^{[3
]}

Huang, Aimin ^{[1
]}

Liu, Jingfeng ^{[4
]}

机构：

[1] Fujian Med Univ, Dept Pathol, Fuzhou 350004, Fujian, Peoples R China

[2] Fujian Med Univ, Div Thorac Surg, Affiliated Hosp 1, Fuzhou 350004, Fujian, Peoples R China

[3] Fujian Med Univ, Shengli Clin Med Coll, Fuzhou 350004, Fujian, Peoples R China

[4] Fujian Med Univ, Div Hepatobiliary Surg, Hepat Dis Ctr, Affiliated Hosp 1, Fuzhou 350005, Fujian, Peoples R China

来源：

MOLECULAR BIOLOGY REPORTS | 2012年 / 39卷 / 06期

关键词：

Dendritic cells; Transforming growth factor-beta1; Interleukin-10; Immune tolerance; REGULATORY T-CELLS; GROWTH-FACTOR-BETA; TGF-BETA; ANTIGEN; IMMUNOSUPPRESSION; DYSFUNCTION; EXPRESSION; GENERATION; TOLERANCE; THERAPY;

D O I：

10.1007/s11033-012-1468-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Dendritic cells (DC) have important functions in T cell immunity and T cell tolerance. Previous studies suggest that immature dendritic cells (imDCs) might be involved in the induction of peripheral T cell tolerance. While interleukin-10 (IL-10) functions at different levels of the immune response, transforming growth factor-beta 1 (TGF-beta 1) is considered to be a key factor in immune tolerance. In this study, we investigated the effects of immature DC (imDC) co-transfected with IL-10 and TGF-beta 1 genes (IL-10-TGF-beta 1-imDC) on inducing immune tolerance. Moreover, we compared the effects of IL-10-TGF-beta 1-imDC with IL-10 transfected imDC (IL-10-imDC) and TGF-beta 1-transfected imDC (TGF-beta 1-imDC), respectively. IL-10-TGF-beta 1-imDC resulted in the down-regulation of MHC class II, CD80 and CD86. IL-10-TGF-beta 1-imDC could induce T cell hyporesponsiveness, and was reluctant to proliferate. IL-10-TGF-beta 1-imDC was more effective than IL-10-imDC and TGF-beta 1-imDC, respectively. In summary, co-expression of IL-10 and TGF-beta 1 affected the immunity of imDCs and enhanced their tolerogenicity. It might be a promising therapy for donor-specific tolerance after organ transplantation.

引用

页码：6633 / 6639

页数：7

共 26 条

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