Efficient multi-enzymatic synthesis of D-xylulose 5-phosphate

被引：65

作者：

Zimmermann, FT

Schneider, A

Schörken, U

Sprenger, GA

Fessner, WD

机构：

[1] Rhein Westfal TH Aachen, Inst Organ Chem, D-52056 Aachen, Germany

[2] Forschungszentrum Julich, Inst Biotechnol 1, D-52425 Julich, Germany

来源：

TETRAHEDRON-ASYMMETRY | 1999年 / 10卷 / 09期

关键词：

D O I：

10.1016/S0957-4166(99)00166-4

中图分类号：

O61 [无机化学];

学科分类号：

070301 ; 081704 ;

摘要：

Pure D-threo-2-pentulose 5-phosphate, a valuable substrate required for enzymatic assays, is prepared in gram-quantities (82% overall yield) by an enzymatic one-pot procedure starting from the readily available precursors hydroxypyruvate and fructose 1,6-bisphosphate. The procedure is based on a stereospecific C-C bond formation catalyzed by the recombinant transketolase A from Escherichia coli. (C) 1999 Elsevier Science Ltd. All rights reserved.

引用

页码：1643 / 1646

页数：4

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