Separation of nucleoside mono-, di- and triphosphates by capillary electrophoresis via cadmium complexation

被引:6
作者
Cahours, X [1 ]
Morin, P [1 ]
Dreux, M [1 ]
机构
[1] Univ Orleans, CNRS, UPRES A 6005, Inst Chim Organ & Analyt, F-45067 Orleans 2, France
关键词
capillary electrophoresis; nucleotides; cadmium complexation;
D O I
10.1007/BF02467610
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The CE separation of twelve nucleotides (5'-mono-, di-, triphosphates of adenosine, guanosine, cytidine and uridine) was improved by adding cadmium ion to the ammonium citrate/citric acid buffer (pH 5, ionic strength 100 mM). Cadmium ion acts as a complexing agent for some nucleotides (ATP, CTP, GTP, UTP, GDP). In order to accelerate the separation, the electroosmotic flow was reversed by flushing the fused-silica capillary with 0.2 % aqueous solution of the polycationic surfactant hexadimethrine bromide. A good separation of the twelve nucleotides studied was then achieved on a dynamically coated capillary in less than 5 min by using an ammonium citrate/citric acid buffer (pH 5, ionic strength 100 mM) to which 2 mM cadmium ion has been added. High peak efficiencies were obtained (210 000 theoretical plates) and the resolution between two adjacent peaks was always greater than 1.5.
引用
收藏
页码:379 / 384
页数:6
相关论文
共 32 条
[1]   HIGH-VOLTAGE CAPILLARY ZONE ELECTROPHORESIS - OPERATING PARAMETERS EFFECTS ON ELECTROENDOSMOTIC FLOWS AND ELECTROPHORETIC MOBILITIES [J].
ALTRIA, KD ;
SIMPSON, CF .
CHROMATOGRAPHIA, 1987, 24 :527-532
[2]  
BIANCHI V, 1983, TOXICOLOGY, V25, P13
[3]   Capillary electrophoretic study of the complexation of nucleotides with magnesium and calcium ions [J].
Cahours, X ;
Morin, P ;
Dreux, M .
CHROMATOGRAPHIA, 1998, 48 (11-12) :739-744
[4]   THE ROLES OF ATP4- AND MG-2+ IN CONTROL STEPS OF PHOSPHOGLYCERATE KINASE [J].
FAIRBROTHER, WJ ;
GRAHAM, HC ;
WILLIAMS, RJP .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1990, 190 (02) :407-414
[5]   Optimization for the separation of ribonucleotides by capillary electrophoresis at high pH [J].
Geldart, SE ;
Brown, PR .
JOURNAL OF CHROMATOGRAPHY A, 1997, 792 (1-2) :67-73
[6]  
GIANNARIS PA, 1993, NUCLEIC ACIDS RES, V21, P2742
[7]   THERMOSPRAY LIQUID-CHROMATOGRAPHIC MASS-SPECTROMETRIC ANALYSIS OF ANTI-AIDS NUCLEOSIDES - QUANTIFICATION OF 2',3'-DIDEOXYCYTIDINE IN PLASMA SAMPLES [J].
JAJOO, HK ;
BENNETT, SM ;
KORNHAUSER, DM .
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1992, 577 (02) :299-304
[8]  
Kuhn R., 1993, capillary electrophoresis: principles and practice, DOI DOI 10.1007/978-3-642-78058-5
[9]   2',3'-DIDEOXYINOSINE (DDI) IN PATIENTS WITH THE ACQUIRED-IMMUNODEFICIENCY-SYNDROME OR AIDS-RELATED COMPLEX - A PHASE-I TRIAL [J].
LAMBERT, JS ;
SEIDLIN, M ;
REICHMAN, RC ;
PLANK, CS ;
LAVERTY, M ;
MORSE, GD ;
KNUPP, C ;
MCLAREN, C ;
PETTINELLI, C ;
VALENTINE, FT ;
DOLIN, R .
NEW ENGLAND JOURNAL OF MEDICINE, 1990, 322 (19) :1333-1340
[10]  
Li S.F. Y., 1992, Capillary Electrophoresis