The structure of the flock house virus B2 protein, a viral suppressor of RNA interference, shows a novel mode of double-stranded RNA recognition

被引：108

作者：

Lingel, A ^{[1
]}

Simon, B ^{[1
]}

Izaurralde, E ^{[1
]}

Sattler, M ^{[1
]}

机构：

[1] European Mol Biol Lab, D-69117 Heidelberg, Germany

来源：

EMBO REPORTS | 2005年 / 6卷 / 12期

关键词：

RNAi; viral suppressor; dsRNA recognition;

D O I：

10.1038/sj.embor.7400583

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the structure of the flock house virus B2 protein, a potent suppressor of RNA interference (RNAi) in animals and plants. The B2 protein is a homodimer in solution and contains three alpha-helices per monomer. Chemical shift perturbation shows that an antiparallel arrangement of helices (alpha 2/alpha 2') forms an elongated binding interface with double-stranded RNA (dsRNA). This implies a novel mode of dsRNA recognition and provides insights into the mechanism of RNAi suppression by B2.

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收藏

页码：1149 / 1155

页数：7

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