Salivary lactoferrin and low-Mr mucin MG2 in Actinobacillus actinomycetemcomitans-associated periodontitis

被引:62
作者
Groenink, J
Walgreen-Weterings, E
Nazmi, K
Bolscher, JGM
Veerman, ECI
van Winkelhoff, AJ
Amerongen, AVH
机构
[1] Vrije Univ Amsterdam, Dept Oral Biochem, Fac Med, Acad Ctr Dent Amsterdam, NL-1081 BT Amsterdam, Netherlands
[2] Acad Ctr Dent Amsterdam, Dept Oral Microbiol, NL-1081 BT Amsterdam, Netherlands
关键词
Actinobacillus actinomycetemcomitans; lactoferrin; mucin; periodontitis; saliva;
D O I
10.1034/j.1600-051X.1999.260501.x
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Concentrations and output of lactoferrin and of low-M-r mucin MG2 were determined in saliva of subjects suffering from Actinobacillus actinomycetem-comitans-associated periodontal disease and healthy subjects. Periodontal patients were clinically examined and a microbiological sample was taken from the deepest bleeding pockets in each quadrant. The number of viable A. actinomycetemcomitans was determined in the sampled sites of each patient. The MG2 output in the diseased subjects (13.6 mu g protein/min) was decreased at least by a factor three compared to periodontal healthy subjects (44.3 mu g protein/min). On the other hand, output of lactoferrin was not significantly different in healthy (9.5 mu g/min) and diseased subjects (7.6 mu g/min). Western analyses demonstrated a higher iron-saturation of lactoferrin in diseased subjects in comparison with the healthy subjects. Lactoferrin degrading enzymes, probably derived from microbial sources, could be detected in saliva of the periodontally diseased subjects, but not in saliva of healthy subjects. The combination of iron-saturation and degradation of lactoferrin suggests that anti-microbial properties of lactoferrin are diminished in periodontitis patients. Moreover, the low concentration of mucin MG2 suggests a decline in mucin defence and consequently a higher susceptibility for oral infection. A negative correlation (r = -0.4, p< 0.05) between the number of subgingival A. actinomycetemcomitans and lactoferrin in saliva suggested that low concentrations of lactoferrin favour the growth of the bacterium. These data indicate that a decline in the salivary defence system might increase the risk for oral infection by A. actinomycetemcomitans.
引用
收藏
页码:269 / 275
页数:7
相关论文
共 37 条
[21]   ADHESION OF ACTINOBACILLUS-ACTINOMYCETEMCOMITANS TO A HUMAN ORAL CELL-LINE [J].
MINTZ, KP ;
FIVESTAYLOR, PM .
INFECTION AND IMMUNITY, 1994, 62 (09) :3672-3678
[22]   THE FUNGICIDAL EFFECT OF HUMAN LACTOFERRIN ON CANDIDA-ALBICANS AND CANDIDA-KRUSEI [J].
NIKAWA, H ;
SAMARANAYAKE, LP ;
TENOVUO, J ;
PANG, KM ;
HAMADA, T .
ARCHIVES OF ORAL BIOLOGY, 1993, 38 (12) :1057-1063
[23]  
PIOTROWSKI J, 1992, BIOCHEM INT, V28, P1021
[24]   ANTIGENS OF ACTINOBACILLUS-ACTINOMYCETEMCOMITANS RECOGNIZED BY PATIENTS WITH JUVENILE PERIODONTITIS AND PERIODONTALLY NORMAL SUBJECTS [J].
SIMS, TJ ;
MONCLA, BJ ;
DARVEAU, RP ;
PAGE, RC .
INFECTION AND IMMUNITY, 1991, 59 (03) :913-924
[25]   BACTEROIDES-GINGIVALIS, BACTEROIDES-INTERMEDIUS AND ACTINOBACILLUS-ACTINOMYCETEMCOMITANS IN HUMAN PERIODONTAL-DISEASES [J].
SLOTS, J ;
LISTGARTEN, MA .
JOURNAL OF CLINICAL PERIODONTOLOGY, 1988, 15 (02) :85-93
[26]   SELECTIVE MEDIUM FOR ISOLATION OF ACTINOBACILLUS-ACTINOMYCETEMCOMITANS [J].
SLOTS, J .
JOURNAL OF CLINICAL MICROBIOLOGY, 1982, 15 (04) :606-609
[27]  
Soderstrom T, 1990, J Parodontol, V9, P195
[28]  
SOUKKA T, 1991, SCAND J DENT RES, V99, P390
[29]  
SOUKKA T, 1992, FEMS MICROBIOL LETT, V69, P223
[30]   SURVIVAL OF HUMAN DENTAL PLAQUE FLORA IN VARIOUS TRANSPORT MEDIA [J].
SYED, SA ;
LOESCHE, WJ .
APPLIED MICROBIOLOGY, 1972, 24 (04) :638-+