Non-covalent assembly of meso-tetra-4-pyridyl porphine with single-stranded DNA to form nano-sized complexes with hydrophobicity-dependent DNA release and anti-tumor activity

被引：15

作者：

Ghosh, Supratim ^{[1
,2
]}

Ucer, Kamil B. ^{[7
]}

D'Agostino, Ralph, Jr. ^{[6
]}

Grant, Ken ^{[3
]}

Sirintrapun, Joseph ^{[3
]}

Thomas, Michael J. ^{[4
]}

Hantgan, Roy ^{[4
]}

Bharadwaj, Manish ^{[5
]}

Gmeiner, William H. ^{[1
,2
,6
]}

机构：

[1] Wake Forest Sch Med, Program Mol Genet, Winston Salem, NC 27157 USA

[2] Wake Forest Sch Med, Dept Canc Biol, Winston Salem, NC 27157 USA

[3] Wake Forest Sch Med, Dept Pathol, Winston Salem, NC 27157 USA

[4] Wake Forest Sch Med, Dept Biochem, Winston Salem, NC 27157 USA

[5] Wake Forest Sch Med, Dept Genrontol, Winston Salem, NC 27157 USA

[6] Wake Forest Sch Med, Ctr Comprehens Canc, Winston Salem, NC 27157 USA

[7] Wake Forest Univ, Dept Phys, Winston Salem, NC 27109 USA

来源：

NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE | 2014年 / 10卷 / 02期

关键词：

Multi-modality nanoparticle; Cancer therapy; Photodynamic therapy; Porphyrin: DNA assembly; WATER-SOLUBLE PORPHYRINS; PHOTODYNAMIC THERAPY; LIPID-PEROXIDATION; CANCER-CELLS; CARBON NANOTUBES; VISIBLE-LIGHT; NANOPARTICLES; PROBE; C-11-BODIPY581/591; PHOTOSENSITIZERS;

D O I：

10.1016/j.nano.2013.07.019

中图分类号：

TB3 [工程材料学];

学科分类号：

082905 [生物质能源与材料];

摘要：

DNA and porphyrin based therapeutics are important for anti-cancer treatment. The present studies demonstrate single-stranded DNA (ssDNA) assembles with meso-tetra-4-pyridyl porphine (MTP) forming porphyrin: DNA nano-complexes (PDN) that are stable in aqueous solution under physiologically relevant conditions and undergo dissociation with DNA release in hydrophobic environments, including cell membranes. PDN formation is DNA-dependent with the ratio of porphyrin: DNA being approximately two DNA nucleobases per porphyrin. PDN produce reactive oxygen species (ROS) in a light-dependent manner under conditions that favor nano-complex dissociation in the presence of hydrophobic solvents. PDN induce light-dependent cytotoxicity in vitro and anti-tumor activity towards bladder cancer xenografts in vivo. Light-dependent, PDN-mediated cell death results from ROS-mediated localized membrane damage due to lipid peroxidation with mass spectrometry indicating the generation of the lipid peroxidation products 9- and 13-hydroxy octadecanoic acid. Our results demonstrate that PDN have properties useful for therapeutic applications, including cancer treatment. (C) 2014 Elsevier Inc. All rights reserved.

引用

页码：451 / 461

页数：11

共 58 条

[1]

Nanofibers Formed Through π ... π Stacking of the Complexes of Glucosyl-C2-salicyl-imine and Phenylalanine: Characterization by Microscopy, Modeling by Molecular Mechanics, and Interaction by α-Helical and β-Sheet Proteins [J].

Acharya, Amitabha ;

Ramanujam, Balaji ;

Mitra, Atanu ;

Rao, Chebrolu P. .

ACS NANO, 2010, 4 (07) :4061-4073

[2]

Necrosis-like death with plasma membrane damage against cervical cancer cells by photodynamic therapy [J].

Ahn, WS ;

Bae, SM ;

Huh, SW ;

Lee, JM ;

Namkoong, SE ;

Han, SJ ;

Kim, CK ;

Kim, JK ;

Kim, YW .

INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER, 2004, 14 (03) :475-482

[3]

Oncologic photodynamic therapy photosensitizers: A clinical review [J].

Allison, Ron R. ;

Sibata, Claudio H. .

PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY, 2010, 7 (02) :61-75

[4]

Biomodulatory approaches to photodynamic therapy for solid tumors [J].

Anand, Sanjay ;

Ortel, Bernhard J. ;

Pereira, Stephen P. ;

Hasan, Tayyaba ;

Maytin, Edward V. .

CANCER LETTERS, 2012, 326 (01) :8-16

[5]

The stability of DNA-porphyrin complexes in the presence of Mn(II) ions [J].

Ananyan, G. ;

Avetisyan, A. ;

Aloyan, L. ;

Dalyan, Y. .

BIOPHYSICAL CHEMISTRY, 2011, 156 (01) :96-101

[6]

[Anonymous], CHEMOTHER RES PRACT

[7]

Effect of bafilomycin A1 and nocodazole on endocytic transport in HeLa cells: Implications for viral uncoating and infection [J].

Bayer, N ;

Schober, D ;

Prchla, E ;

Murphy, RF ;

Blaas, D ;

Fuchs, R .

JOURNAL OF VIROLOGY, 1998, 72 (12) :9645-9655

[8]

Template-imprinted chiral porphyrin aggregates [J].

Bellacchio, E ;

Lauceri, R ;

Gurrieri, S ;

Scolaro, LM ;

Romeo, A ;

Purrello, R .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1998, 120 (47) :12353-12354

[9]

A DNA-porphyrin minor-groove complex at atomic resolution: The structural consequences of porphyrin ruffling [J].

Bennett, M ;

Krah, A ;

Wien, F ;

Garman, E ;

Mckenna, R ;

Sanderson, M ;

Neidle, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (17) :9476-9481

[10]

Investigation of oxidation in freeze-dried membranes using the fluorescent probe C11-BODIPY581/591 [J].

Carlsen, Charlotte U. ;

Kurtmann, Lone ;

Bruggemann, Dagmar A. ;

Hoff, Signe ;

Risbo, Jens ;

Skibsted, Leif H. .

CRYOBIOLOGY, 2009, 58 (03) :262-267

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