Construction of green fluorescent protein reporter genes for genotoxicity test (SOS/umu-Test) and improvement of mutagen-sensitivity

被引：15

作者：

Arai, R

Makita, Y

Oda, Y

Nagamune, T ^{[1
]}

机构：

[1] Univ Tokyo, Grad Sch Engn, Dept Chem & Biotechnol, Bunkyo Ku, Tokyo 1138656, Japan

[2] Osaka Prefectural Inst Publ Hlth, Higashinari Ku, Osaka 5370025, Japan

来源：

JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2001年 / 92卷 / 03期

关键词：

umu-test; green fluorescent protein (GFP); genotoxicity; tandem promoter; chimeric promoter; recA mutant;

D O I：

10.1263/jbb.92.301

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Green fluorescent protein (GFP) reporter genes for the bacterial umu-test were constructed. Utilization of tandem, lacUV5 and chimeric trp/umu promoters, and coexpression of the Escherichia coli recA5327 mutant enhanced the GFP expression level fourteen-fold over that of the system with only the umu promoter, thereby improving the sensitivity of the umu-test.

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收藏

页码：301 / 304

页数：4

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