Construction of a umuC′-luxAB plasmid for the detection of mutagenic DNA repair via luminescence

被引：15

作者：

Justus, T ^{[1
]}

Thomas, SM ^{[1
]}

机构：

[1] Flinders Univ S Australia, Sch Biol Sci, Adelaide, SA 5001, Australia

来源：

MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS | 1998年 / 398卷 / 1-2期

基金：

澳大利亚研究理事会;

关键词：

E-coli; mutagenicity; umuDC; luxAB;

D O I：

10.1016/S0027-5107(97)00215-7

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

This paper describes a novel system for the detection of mutagenic DNA repair in Escherichia coli. The DNA damage inducible umuC gene of Escherichia coli has been fused to the luxAB genes from Vibrio harveyi that encode the enzyme luciferase. Mutagenicity has been assessed semi-quantitatively by the induction of bioluminescence. This system is simple, rapid and equivalent in sensitivity to other currently available test procedures. Its use in the detection of known SOS mutagens MMS, MNNG and UV is described. (C) 1998 Elsevier Science B.V.

引用

页码：131 / 141

页数：11

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