Phase-contrast X-ray microtomography of mouse fetus

被引：25

作者：

Hoshino, Masato ^{[1
]}

Uesugi, Kentaro ^{[1
]}

Yagi, Naoto ^{[1
]}

机构：

[1] SPring 8, Japan Synchrotron Radiat Res Inst, Sayo, Hyogo 6795198, Japan

来源：

BIOLOGY OPEN | 2012年 / 1卷 / 03期

关键词：

Phase-contrast; X-ray CT; Fetus; VIRTUAL HISTOLOGY; TOMOGRAPHY; EMBRYOS; TOOLS;

D O I：

10.1242/bio.2012430

中图分类号：

Q [生物科学];

学科分类号：

090105 [作物生产系统与生态工程];

摘要：

A phase-contrast X-ray microtomography system using the Talbot imaging has been built at the SPring-8 synchrotron radiation facility. This system has much higher density resolution than absorption-based X-ray microtomography. The tomographic sections of formalin-fixed mouse fetuses obtained with this method clearly depict various organs without any staining at a pixel resolution of up to 5 mm. Since this technique allows us to obtain three-dimensional structural information without sectioning, it will be particularly useful to examine anomalies that take place during development. It can be also used to quantitatively measure volume and mass of organs during development. (C) 2012. Published by The Company of Biologists Ltd.

引用

页码：269 / 274

页数：6

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