Innovation in detection of microparticles and exosomes

被引：206

作者：

van der Pol, E. ^{[1
,2
]}

Coumans, F. ^{[1
,2
]}

Varga, Z. ^{[3
]}

Krumrey, M. ^{[4
]}

Nieuwland, R. ^{[1
]}

机构：

[1] Univ Amsterdam, Acad Med Ctr, Lab Expt Clin Chem, NL-1105 AZ Amsterdam, Netherlands

[2] Univ Amsterdam, Acad Med Ctr, NL-1105 AZ Amsterdam, Netherlands

[3] Hungarian Acad Sci, Inst Mol Pharmacol, Res Ctr Nat Sci, Budapest, Hungary

[4] Phys Tech Bundesanstalt, Berlin, Germany

来源：

JOURNAL OF THROMBOSIS AND HAEMOSTASIS | 2013年 / 11卷

关键词：

exosomes; flow cytometry; microparticles; raman spectroscopy; secretory vesicles; SIZE DISTRIBUTION MEASUREMENTS; DYNAMIC LIGHT-SCATTERING; ATOMIC-FORCE MICROSCOPY; CALIBRATED POLYSTYRENE BEADS; CELL-DERIVED MICROPARTICLES; X-RAY-SCATTERING; FLOW-CYTOMETRY; CIRCULATING MICROPARTICLES; PLATELET MICROPARTICLES; IMMUNE-COMPLEXES;

D O I：

10.1111/jth.12254

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Cell-derived or extracellular vesicles, including microparticles and exosomes, are abundantly present in body fluids such as blood. Although such vesicles have gained strong clinical and scientific interest, their detection is difficult because many vesicles are extremely small with a diameter of less than 100nm, and, moreover, these vesicles have a low refractive index and are heterogeneous in both size and composition. In this review, we focus on the relatively high throughput detection of vesicles in suspension by flow cytometry, resistive pulse sensing, and nanoparticle tracking analysis, and we will discuss their applicability and limitations. Finally, we discuss four methods that are not commercially available: Raman microspectroscopy, micro nuclear magnetic resonance, small-angle X-ray scattering (SAXS), and anomalous SAXS. These methods are currently being explored to study vesicles and are likely to offer novel information for future developments.

引用

页码：36 / 45

页数：10

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