Preliminary contribution to the quantification of HMF in honey by electrochemical biosensor chips

Several hydroximethylfurfural (HMF) microbiosensors, based on a microelectrode configuration built by photolithographic techniques, have been developed. Two different biosensors based on the aldehyde dehydrogenase (ALDH) enzyme coupling with NADH oxidase (NOD) or diaphorase (Diaph) are described. Experimental variables were optimized using experimental design methodology, by central composite designs, taking the intensity registered for a given HMF solution as response. Given the reproducibility (residual standard deviation (RSD), 7.20%), repeatability (RSD = 3%) and limit of detection (LOD) = 1.68 x 10(-4) mol dm(-3) (alpha = beta = 0.05 and a replicate) reached by the ALDH-Diaph microbiosensor under the optimum conditions, the analysis of HMF in honey was successfully accomplished.