Electrochemical DNA Biosensor Based on Graphene and TiO2 Nanorods Composite Film for the Detection of Transgenic Soybean Gene Sequence of MON89788

被引：30

作者：

Gao, Hongwei ^{[2
]}

Sun, Min ^{[2
]}

Lin, Chao ^{[2
]}

Wang, Shubai ^{[1
]}

机构：

[1] Qingdao Agr Univ, Coll Anim Sci & Vet Med, Qingdao, Peoples R China

[2] Shandong Entry Exit Inspect & Quarantine Bur Peop, Qingdao, Peoples R China

来源：

ELECTROANALYSIS | 2012年 / 24卷 / 12期

关键词：

TiO2; nanorods; Graphene; Chitosan; Carbon ionic liquid electrode; Electrochemical DNA biosensor; IONIC LIQUID ELECTRODE; VOLTAMMETRIC DETECTION; GOLD NANOPARTICLES; AU NANOPARTICLES; METHYLENE-BLUE; CARBON; HYBRIDIZATION; SENSOR; NANOTUBES; DOPAMINE;

D O I：

10.1002/elan.201200403

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Based on graphene (GR), TiO2 nanorods, and chitosan (CTS) nanocomposite modified carbon ionic liquid electrode (CILE) as substrate electrode, a new electrochemical DNA biosensor was effectively fabricated for the detection of the transgenic soybean sequence of MON89788. By using methylene blue (MB) as hybridization indicator for monitoring the hybridization with different ssDNA sequences, the differential pulse voltammetric response of MB on DNA modified electrodes were recorded and compared. Due to the synergistic effects of TiO2 nanorods and GR on the electrode surface, the electrochemical responses of MB were greatly increased. Under optimal conditions the differential pulse voltammetric response of the target ssDNA sequence could be detected in the range from 1.0x10-12 to 1.0x10-6 mol/L with a detection limit of 7.21x10-13 mol/L (3s). This electrochemical DNA biosensor was further applied to the polymerase chain reaction (PCR) product of transgenic soybeans with satisfactory results.

引用

页码：2283 / 2290

页数：8

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