Amperometric immunosensors based on rigid conducting immunocomposites

被引：114

作者：

Santandreu, M ^{[1
]}

Cespedes, F ^{[1
]}

Alegret, S ^{[1
]}

MartinezFabregas, E ^{[1
]}

机构：

[1] UNIV AUTONOMA BARCELONA, DEPT QUIM, GRP SENSORS & BIOSENSORS, BELLATERRA 08193, CATALONIA, SPAIN

来源：

ANALYTICAL CHEMISTRY | 1997年 / 69卷 / 11期

关键词：

D O I：

10.1021/ac961222b

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Novel polishable immunosensors based on rigid biocomposite materials have been constructed. These biocomposites contain graphite powder, rabbit IgG, and methacrylate or epoxy resins, This material acts as a reservoir for the biological molecules and as a transducer at the same time. In order to study the potential analytical properties of this new type of material, a competitive binding assay was developed to determine the RIgG present in a sample with the aid of goat anti-rabbit IgG labeled with alkaline phosphatase. Using phenyl phosphate as a substrate, the phenol produced by the enzymatic reaction was amperometrically detected at 800 mV (vs Ag/AgCl). The surface of the immunosensor can be regenerated by simply polishing, obtaining fresh immunocomposite ready to be used in a new competitive assay.

引用

页码：2080 / 2085

页数：6

共 30 条

[1] Carbon-polymer biocomposites for amperometric sensing [J].