Transcriptomic analysis of peritoneal cells in a mouse model of sepsis: confirmatory and novel results in early and late sepsis

被引:10
作者
Bhatty, Minny [1 ]
Fan, Ruping [2 ]
Muir, William M. [3 ]
Pruett, Stephen B. [1 ,2 ]
Nanduri, Bindu [1 ]
机构
[1] Mississippi State Univ, Coll Vet Med, Dept Basic Sci, Mississippi State, MS 39762 USA
[2] Louisiana State Univ, Hlth Sci Ctr, Dept Cellular Biol & Anat, Shreveport, LA 71130 USA
[3] Purdue Univ, Dept Anim Sci, W Lafayette, IN 47907 USA
来源
BMC GENOMICS | 2012年 / 13卷
基金
美国国家卫生研究院;
关键词
Intra-abdominal sepsis; Microarray; Peritoneal leukocytes; GENE-EXPRESSION PROFILES; NF-KAPPA-B; ESCHERICHIA-COLI; INFLAMMATORY RESPONSE; ENDOTOXIN CHALLENGE; INNATE IMMUNITY; ANIMAL-MODELS; LUNG; ADRENOMEDULLIN; ETHANOL;
D O I
10.1186/1471-2164-13-509
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Background: The events leading to sepsis start with an invasive infection of a primary organ of the body followed by an overwhelming systemic response. Intra-abdominal infections are the second most common cause of sepsis. Peritoneal fluid is the primary site of infection in these cases. A microarray-based approach was used to study the temporal changes in cells from the peritoneal cavity of septic mice and to identify potential biomarkers and therapeutic targets for this subset of sepsis patients. Results: We conducted microarray analysis of the peritoneal cells of mice infected with a non-pathogenic strain of Escherichia coli. Differentially expressed genes were identified at two early (1 h, 2 h) and one late time point (18 h). A multiplexed bead array analysis was used to confirm protein expression for several cytokines which showed differential expression at different time points based on the microarray data. Gene Ontology based hypothesis testing identified a positive bias of differentially expressed genes associated with cellular development and cell death at 2 h and 18 h respectively. Most differentially expressed genes common to all 3 time points had an immune response related function, consistent with the observation that a few bacteria are still present at 18 h. Conclusions: Transcriptional regulators like PLAGL2, EBF1, TCF7, KLF10 and SBNO2, previously not described in sepsis, are differentially expressed at early and late time points. Expression pattern for key biomarkers in this study is similar to that reported in human sepsis, indicating the suitability of this model for future studies of sepsis, and the observed differences in gene expression suggest species differences or differences in the response of blood leukocytes and peritoneal leukocytes.
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页数:13
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