High-performance liquid chromatography of lactose with evaporative light scattering detection, applied to determine fine particle dose of carrier in dry powder inhalation products

被引：22

作者：

Beilmann, B

Langguth, P

Häusler, H

Grass, P

机构：

[1] Univ Mainz, Inst Pharm, D-55099 Mainz, Germany

[2] Boehringer Ingelheim Pharma GMBH & Co KG, D-55216 Ingelheim, Germany

来源：

JOURNAL OF CHROMATOGRAPHY A | 2006年 / 1107卷 / 1-2期

关键词：

lactose; evaporative light scattering detection; aminopropyl column; dry powder inhalation products;

D O I：

10.1016/j.chroma.2005.12.083

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for quantification of the fine particle dose of lactose is described, using a hydrophilic interaction chromatography (HILIC) method and evaporative light scattering detection. The HILIC method used an aminopropyl column and a mobile phase consisting of acetonitril/water (80/20, v/v) for isocratic elution. Sensitive chromatography was obtained using a low concentration of water in the extraction solvent. The detection limit (RSD < 10%) at an injection volume of 10 mu L is 10 mu g/mL. Linearity was obtained in the range of 10-80 mu g/mL (R-2 > 0.99). A relative standard deviation (RSD) of 0.5% (N = 6) demonstrated good precision of the optimized method. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：204 / 207

页数：4

共 9 条

[1] STUDY OF THE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION OF REDUCING SUGARS, APPLIED TO THE DETERMINATION OF LACTOSE IN MILK [J].