High-performance liquid chromatographic analysis of Peptide T in rabbit plasma with on-line column enrichment

被引：8

作者：

Bechgaard, E ^{[1
]}

Bagger, M ^{[1
]}

Larsen, R ^{[1
]}

Nielsen, HW ^{[1
]}

机构：

[1] PEPTECH EUROPE AS,DK-3400 HILLEROD,DENMARK

来源：

JOURNAL OF CHROMATOGRAPHY B | 1997年 / 693卷 / 01期

关键词：

peptide T;

D O I：

10.1016/S0378-4347(96)00537-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The present paper describes the development of a simple and sensitive analytical method for quantification of Peptide T (PT) in rabbit plasma, using standard analytical equipment and on-line column enrichment, without prior extraction, clean-up or derivatization. The analytical procedure was found to be accurate, precise and linear. The accuracy was 100% (range 97-103%) and the mean precision was 8% (range 3-14%) for all (n=6) concentrations (0, 15, 50, 100 and 200 ng/ml). The total recovery was found to be approximately 80%, and it was found to be dependent upon the injection rate onto the extraction column. The correlation between added and found concentrations was 0.9982, and the limit of detection was estimated to be around 5 ng/ml. The method is therefore found to be suitable for bioavailability studies, involving Peptide T, in rabbits.

引用

页码：237 / 240

页数：4

共 8 条

[1] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF AN ARGININE-CONTAINING OCTAPEPTIDE ANTAGONIST OF VASOPRESSIN IN HUMAN PLASMA BY MEANS OF A SELECTIVE POSTCOLUMN REACTION WITH FLUORESCENCE DETECTION [J].