A Bacillus megaterium plasmid system for the production, export, and one-step purification of affinity-tagged heterologous levansucrase from growth medium

被引：40

作者：

Malten, M

Biedendieck, R

Gamer, M

Drews, AC

Stammen, S

Buchholz, K

Dijkhuizen, L

Jahn, D

机构：

[1] Tech Univ Carolo Wilhelmina Braunschweig, Inst Microbiol, D-38106 Braunschweig, Germany

[2] Inst Tech Chem, D-38106 Braunschweig, Germany

[3] Univ Groningen, Groningen Biomol Sci & Biotechnol Inst, GBB, CCB,TNO Qual Life, NL-9751 NN Haren, Netherlands

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2006年 / 72卷 / 02期

关键词：

D O I：

10.1128/AEM.72.2.1677-1679.2006

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A multiple vector system for the production and export of recombinant affinity-tagged proteins in Bacillus megaterium was developed. Up to 1 mg/liter of a His(6)-tagged or Strep-tagged Lactobacillus reuteri levansucrase was directed into the growth medium, using the B. megaterium esterase LipA signal peptide, and recovered by one-step affinity chromatography.

引用

页码：1677 / 1679

页数：3

共 7 条

[1] Coexpression of the type I signal peptidase gene sipM increases recombinant protein production and export in Bacillus megaterium MS941 [J].