Clinical significance of telomerase activity in the non-cancerous epithelial region of oesophageal squamous cell carcinoma

被引：15

作者：

Koyanagi, K ^{[1
]}

Ozawa, S ^{[1
]}

Ando, N ^{[1
]}

Takeuchi, H ^{[1
]}

Ueda, M ^{[1
]}

Kitajima, M ^{[1
]}

机构：

[1] Keio Univ, Sch Med, Dept Surg, Shinjuku Ku, Tokyo 1608582, Japan

来源：

BRITISH JOURNAL OF SURGERY | 1999年 / 86卷 / 05期

关键词：

D O I：

10.1046/j.1365-2168.1999.01094.x

中图分类号：

R61 [外科手术学];

学科分类号：

摘要：

Background: The aim of this study was to examine telomerase activity in affected and adjacent tissue in patients with oesophageal squamous cell carcinoma (SCC). Methods: Telomerase activity was measured in oesophageal SCC cells, normal oesophageal culture cells, primary cancer tissues and adjacent normal tissues from patients with oesophageal SCC using a polymerase chain reaction-based assay. Results: All oesophageal SCC cells had telomerase activity, whereas normal cultured cells showed no activity. All 57 cancer tissues showed telomerase activity compared with only five (10 per cent) of 50 normal tissues. Cancer cells infiltrating the vessels of mucosal or submucosal tissues in non-cancerous regions were detected in four of five telomerase-positive normal tissues, whereas such infiltration was detected in only three of 45 telomerase-negative normal tissues. Conclusion: In patients with oesophageal SCC, measurement of telomerase activity in normal epithelium is a highly sensitive method of detecting the microinvasion of cancer cells.

引用

页码：674 / 679

页数：6

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