Prime-boost vaccination using cysteine proteinases type I and II of Leishmania infantum confers protective immunity in murine visceral leishmaniasis

被引：74

作者：

Rafati, S

Zahedifard, F

Nazgouee, F

机构：

[1] Inst Pasteur, Mol Immunol & Vaccine Res Lab, Tehran, Iran

[2] Inst Pasteur, Clin Res Unit, Tehran, Iran

来源：

VACCINE | 2006年 / 24卷 / 12期

关键词：

cysteine proteinase type I and II; prime-boost vaccination; Leishmania infantum;

D O I：

10.1016/j.vaccine.2005.11.011

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Vaccination with a cocktail of DNA encoding cysteine proteinases has been previously shown to confer protection against experimental cutaneous leishmaniasis (CL). In the present study we test the efficacy of immunization against Leishmania infantum in a murine model of infection, using a prime-boost strategy. BALB/c mice were immunized twice, in a 3 weeks interval, with cocktail of plasmids DNA encoding type I (cpb) and II (cpa) cysteine proteinases. DNA immunization was then followed by a boost with rCPA/rCPB in addition to CpG ODN and Montanide720 as adjuvant. Analysis of the immune response showed that vaccination mainly elicited antigen-specific IgG2a antibodies, suggesting the induction of a Th1 immune response. This was further confirmed by the analysis of the splenic cytokine production: at all time points the ratio of IFN-gamma/IL-5 induced upon restimulation with rCPA and rCPB was always significantly higher in vaccinated group compared to both control groups. (c) 2005 Elsevier Ltd. All rights reserved.

引用

页码：2169 / 2175

页数：7

共 33 条

[21] Expression of cysteine proteinase type I and II of Leishmania infantum and their recognition by sera during canine and human visceral leishmaniasis [J].