Capillary electrophoretic study of cisplatin interaction with nucleoside monophosphates, di- and trinucleotides

被引:34
作者
Zenker, A
Galanski, MS
Bereuter, TL
Keppler, BK [1 ]
Lindner, W
机构
[1] Inst Gen & Inorgan Chem, Wahringer Str 42, A-1090 Vienna, Austria
[2] Univ Vienna, Inst Analyt Chem, A-1090 Vienna, Austria
关键词
kinetic studies; pharmaceutical analysis; cisplatin; nucleoside monophosphates; nucleotides;
D O I
10.1016/S0021-9673(99)00467-7
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
cis-Diamminedichloroplatinum(II) (cisplatin) is applied against different kinds of cancer although toxic side effects are known. Screening systems for alternative compounds with higher effectiveness but minimizing toxic side effects are required. We investigated the adduct formation of cisplatin with nucleoside monophosphates, di- and trinucleotides. Capillary electrophoretic separations were performed in a sodium phosphate buffer using an instrument equipped with a diode array detector. Adduct formation results in a significant shift of lambda(max) to lower energy compared to free nucleotides. Therefore, UV spectra are an important tool for peak identification. We could separate and identify all four common nucleotides and their major platinum adducts in a single run demonstrating the suitability of CE for these kinds of investigations. Furthermore, kinetic studies of these reactions are performed. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:337 / 346
页数:10
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